以吖啶橙单体(AO)为荧光探针,CdTe量子点为参比物,利用层层组装技术构建AO和CdTe量子点双荧光自组装多层膜,并考察其组装条件.研究结果表明,CdTe浓度为8.33 mmol·L-1,壳聚糖质量浓度为1.0 g·L-1,AO组装液浓度为10μmol·L-1的组装效果最佳.通过双荧光自组装多层膜的自动校准能力测试,所构建的自组装膜双荧光强度比(AO和CdTe的荧光强度比)不随外界环境的变化而变化.
Using fluorescent dye acridine orange(AO) as a fluorescent probe and CdTe as an internal standard,we assembled AO and CdTe quantum dots on the surface of silanized quartz respectively.The assembled condition have been discussed.The optimal concentration of CdTe is 8.33 mmol·L-1,CS is 1.0 g·L-1 and AO is 10 μmol·L-1.These SAMs can well avoid the disturbance from the environmental factors,such as the power of the equipment,slit ratio,and improve the accuracy of fluorescence analysis.