克否定细菌,命名 JDC-16,能作为碳和精力的唯一的来源在酞酸酸酉旨(PAE ) 的底层上成长很好,从河污泥被孤立。基于 16S rRNA 基因顺序的形态学,生理、生物化学的性质和分析,它 preliminarily 被识别属于类 Acinetobacter。底层利用范围的结果显示种类 JDC-16 能除了 diisononyl phthalate (DINP ) 利用许多 phthalates。把 diethyl phthalate (DEP ) 用作模型混合物的降级测试出现为由 Acinetobacter sp 的 DEP 降级的最佳的 pH 和温度。JDC-16 是 8.0 和 35 吗??
A gram negative bacterium, named JDC-16, which can grow well on the substrate of phthalic acid esters (PAEs) as the sole source of carbon and energy, was isolated from river sludge. Based on the morphology, physiological and biochemical properties and analysis of 16S rRNA gene sequence, it was preliminarily identified belonging to the genus Acinetobacter. The result of substrates utilization range indicates that strain JDC-16 can utilize a variety of phthalates except for diisononyl phthalate (DINP). The degradation tests using diethyl phthalate (DEP) as the model compound show that the optimal pH and temperature for DEP degradation by Acinetobacter sp. JDC-16 is 8.0 and 35 ℃, respectively. Meanwhile, degradation kinetics under various initial concentrations of DEP reveals that substrate depletion curves fit well with the modified Gompertz model with high correlation coefficient (R^2〉0.99). Furthermore, the substrate induction test indicates that DEP-induction can apparently shorten the lag phase and enhance the degradation rate. This work highlights the potential of this isolate for bioremediation of phthalates-contaminated environments.