中文摘要：

用改进的组织印迹方法批量检测黄淮地区69份SMV样品,结果显示42个标样呈现非常清晰的蓝紫色阳性反应,26个标样显色较弱而无法确证,1个标样呈阴性反应。进一步对图片进行600 dpi扫描并放大200倍进行分析,发现26个显色弱的标样中有25个呈阳性反应,1个标样显色很弱仍无法确证,使得检出率提高了37%。利用RT-PCR程序对随机选取的8个样品进行验证,发现纯化与未纯化的RNA模板均能得到较为清晰的扩增条带,而只有纯化后的PCR产物二次扩增才有预期的条带,且双酶切可产生245 bp、255 bp和307 bp 3个预期的片段。表明改进的组织印迹方法能够用于批量、高效地检测SMV,而RT-PCR可用于更为精确的检验。

英文摘要：

With improved tissue blotting approach,69 SMV samples from the Yellow and Huaihe River areas were detected and the results showed that 42 samples presented a very clear blue-violet-positive reaction,26 samples could not made conclusion for weaker color and one sample was negative.Further analysis by 600 dpi scan and enlarging the picture for 200 times,it was observed that 25 from 26 samples suffering from weak color showed positive results,and the negative standard sample were still unable to confirm the color,which makes the detection rate increased by 37%.RT-PCR verification were used in random selected eight samples and the results showed that both purified and unpurified RNA templates could obtain distinct bands,however,only the purified PCR products could be amplified repeatedly with the expected secondary bands,and the double-digestion could also produce three expected fragments,245 bp,255 bp and 307 bp.The results above indicated that the improved tissue blotting method could detect soybean SMV efficiently and bulkily,RT-PCR detection could be used in more precise testing.