中文摘要：

为了解LEC1（Leafy Cotyledon 1）基因对油桐油脂合成过程的调控机理,以油桐种仁转录组数据库中基因的部分c DNA序列为基础,采用RT-PCR技术从油桐种子中克隆LEC1基因的全长c DNA,并对其进行序列分析。油桐LEC1基因全长c DNA为1 152 bp,编码区为729 bp（142~870）,编码243个氨基酸,5′端非编码区和3′端非编码区分别为141 bp和263 bp。该基因编码蛋白质的相对分子质量为27 025.4 Da,理论等电点为6.97;蛋白二级结构以不规则卷曲和α螺旋为主,是不具有跨膜结构的膜外蛋白。经对比发现,油桐LEC1具有典型的HAP3结构特点,在不保守的N端和C端中间有1个十分保守的结构域,与拟南芥、玉米、麻风树、黄连木等的LEC1氨基酸序列高度同源。

英文摘要：

In order to learn regulation mechanism of LEC1 gene（Leafy Cotyledon 1） to tung oil synthesis, the full-length c DNA of LEC1 gene was cloned from Vernicia fordii seeds by RT-PCR method on the basis of a part of c DNA sequences of the gene in transcription database of V. fordii kernel, and the sequences were analyzed. The full-length c DNA of LEC1 gene in V. fordii was 1 152 bp, ORF consisting of 729 bp（142-870） coded 243 amino acid, and 5′ UTR and 3′ UTR were 141 bp and 263 bp respectively. The relative molecular mass of coded protein by LEC1 gene was 27 025.4 Da, and its theoretical isoelectric point was 6.97. The predicted second structure of the protein mainly consisted of random coil and alpha helix, and had no transmembrane domain. The results of phylogenetic investigation revealed that LEC1 in V. fordii had typical HAP3 structure characteristics and had a very conservative structure domain between N end and C end, and the coded protein was highly homologous with those of other species.