中文摘要：

通过微列阵芯片（Microarray）分析,从珠眉海棠盐胁迫cDNA文库中分离得到MzSTO（Salt toleranceprotein）全长基因。结果表明：MzSTO基因cDNA全长1 066bp,开放阅读框共729bp,5′-UTR和3′-UTR的长度分别是49 bp和288 bp;MzSTO编码242个氨基酸,N端有2个保守的B-box锌指结构域（CX2CX8CX7CX2CX4HX8H）;半定量RT-PCR表明MzSTO基因受到盐和干旱胁迫抑制,受冷处理诱导,并响应ABA（abscisic acid）。以上结果表明MzSTO基因可能通过依赖ABA的途径参与非生物逆境胁迫。

英文摘要：

The fuN-length cDNA sequence of MzSTO gene was isolated from a cDNA library of Malus zumi. The full- length cDNA of MzSTO was 1 066 bp. The 5＇-UTR and the 3＇-UTR were 49 and 288 bp respectively. Its open reading frame of 729 bp encoded a protein of 242 amino acids. There were two B-box zinc-finger domains （CX2CX8CX7CX2CX4HXSH） near N-terminal. MzSTO was induced by low temperature stress and repressed by high- salinLty and drought treatment, whLch was, also responsLve to ABA （abscisic acid） treatment by semi-quantative RT- PCR. All the results showed that MzSTO was involved in response of abiotic stresses including salt,drought and cold stresses through a possible ABA-dependent pathway in Malus zumi.