中文摘要：

以羽衣甘蓝（Brassica oleracea var．acephala）SmbSmb自交不亲和系为试验材料，分离了控制花粉自交不亲和基因——S13-b单倍型富含半胱氨酸基因（SCR13-b），GenBank收录号为EF577028。SCR13-bcDNA序列含有237bp的开放读码框（ORF），编码一个含78个氨基酸的蛋白。SCR13-b sDNA含有一个758bp的内含子，内含子5’供体位点和3’受体位点边界序列符合GU-AG规则；DNAblot分析表明SCR13-b在基因组中只有单一拷贝；授粉生物活性分析试验显示原核表达SCR13-b融合蛋白能够启动SI反应，抑制杂交亲和花粉的萌发和生长。

英文摘要：

The S13-b-IOcHS cysteine-rich gene （SCR13_b） was isolated from pollen of ornamental kale （Brassica oleracea var. acephala） S13-b homozygotes, GenBank accession number is EF577028. The SCR13-b cDNA sequence had an ORF of 237 bp, encoding a 78-aa protein. The genomic SCR13_b sequence contained a 758 bp intron with boundary sequences of GT at 5＇ site and AG at 3＇ site, which complied with the typical ‘ GT-AG rule’ in plants. Genomic DNA blot analysis showed that SCR13-b was a single copy gene in S13-bS13-b lines. Pollination bioassay showed that prokaryotic expressed recombinant SCR13-b can induce SI response and inhibit cross pollen germination and growth.