中文摘要：

通过改进计时电流法的精确模型数值解仿真，准确分析得到响应电流峰值信号与被测物浓度及电路系统装置相关参数的关系．研究发现，采用改进电路装置检测试剂电化学反应的电流峰值信号，除与试剂浓度有关外，还受试剂温度的影响．而理论模型中能斯特方程和扩散定律包含温度参量，因此数值解还可作为确定电流与温度变化关系的依据．采用改进CA法快速检测试剂温度的变化，有效提高PCR仪测温装置的实时性，并且降低测温的复杂性．

英文摘要：

Based on a numerical model of improved chronoamperometry, the relationship between the working currents and detection reagent concentration was analyzed quantitatively, and the dependence of peak currents on parameters for the improved system was also represented. We found that the cur- rents were not only changed with the reagents concentration, but also were influenced by the tempera- ture during the whole experiments. Both Nernst equation and Flick＇ s law contain temperature, so the numerical model could also be used to find out the law between currents and temperature. Experimen- tal results show that the improved CA model could indicate real - time temperature change of detection reagent. This research efficiently increases the real - time performance of the PCR instrument and de- creases its complexity.