中文摘要：

目的：研究牛蒡子苷与牛血清白蛋白（BSA）的相互作用机制。方法：采用紫外-可见吸收光谱法和同步荧光光谱法。结果：在△A=15nm时，牛蒡子苷对BSA的荧光具有规律性增强作用，最大发射波长蓝移，表明牛蒡子苷的加入影响了BSA中酪氨酸残基的微环境。用荧光效应增强方程计算它们在298K、310K和318K温度下的结合常数分别为K1=7．899×10^4 L／mol、K2=5．962×10^4L/mol和K3=3．903×10^4L／mol，对应温度下的热力学参数△H=-26．874kJ／mol，△S分别为3．601、4．737、3．395J／（mol·K），△G分别为-27．940、-28．340、-27．951kJ／mol。结论：牛血清白蛋白与牛蒡子苷分子间有较强的结合作用，且结合力以静电相互作用为主。

英文摘要：

Objective：To study the interaction between arctiin and bovine serum albumin （BSA）. Methods： Fluorescence spectroscopy and ultra-violet spectroscopy were used. Results： With the increased concentration of arctiin, fluorescence intensity （△λ = 15 nm） increased significantly and the emission spectrum showed a blue shift. The binding constants at 298 K, 308 K and 318 K were 7.899×10^4 L/mol, 5. 962×10^4 L/mol, and 3. 903×10^4 L/mol, respectively; and the thermodynamic parameters were △H=-26. 874 kJ/mol, △S=3. 601, 4. 737, 3. 395 J/（mol·K）, △G = -27. 940, -28. 340, -27. 951 kJ/mol, respectively. Conclusions： The interaction between arctiin and BSA is strong and the main binding force is electrostatic force.