中文摘要：

目的观察水苏糖对猪到人异种心脏移植超急性排斥反应的抑制作用。方法以人血液体外灌注猪游离心脏为基础，模拟猪到人异种心脏移植的超急性排斥反应模型。实验分为A、B两组：即分别用人血液灌注及人血液加水苏糖灌注猪离体心脏。观察灌注后两组心脏的跳动时间；体外灌注1h后，对两组灌注心脏进行免疫组织化学（测定IgG及IgM的沉积）及病理学分析。结果A组灌注心脏平均跳动时间为（9．5±2．5）min；B组灌注心脏平均跳动时间为（46．8±8．1）min，其中有1个心脏在灌注的1h内一直跳动；两组心脏跳动时间比较，差异有统计学意义（P〈0．01）。A组心肌间质呈弥漫性出血、水肿，血管扩张，内皮细胞肿胀、坏死；免疫组织化学检查显示心肌血管内皮组织中有IgG及IgM沉积。B组心肌间质未见出、凝血和坏死，血管内皮细胞未见肿胀；免疫组织化学检测未见IgG及IgM沉积。结论水苏糖对异种心脏移植超急性排斥反应具有抑制作用。

英文摘要：

Objective To study the inhibitory effects of stachyose on the hyperacute rejection in pig-to-human heart xenotransplantation. Methods A pig-to-human xenogeneic heart transplantation model was established based on an in vitro free heart blood perfusion system. The pig hearts were divided into two groups： group A （pig hearts treated with human blood perfusion as control） and group B （pig hearts treated with human blood plus stachyose perfusion）. After perfusion for I h, the heart xenografts were examined for deposit of IgG and IgM by immunohistochemistry and pathological analy sis. Results The mean survival time of perfusion hearts in groups A and B was （9. 5 ± 2. 5） min and （46. 8 ± 8. 1 ） min respectively （P〈0. 01 ）. Histopathological studies revealed that haemorrhagia, edema, necrosis of endothelial cells, distension of blood vessel were found in group A, but in group B no obvious pathological changes were found. Immunohistochemistry studies also indicated there was obvious deposit of IgG and IgM in group A while in group B no deposit of IgG or IgM was found. Conclusion Stachyose can inhibit the hyperacute rejection in pig-human xenotransplantation.