目的 了解广东省广州市鼠及蝙蝠狂犬病毒的携带情况,分析鼠、蝙蝠作为人类狂犬病潜在传染源的可能性。方法 于2013—2015年在广州市公园、医院以及农贸市场捕获褐家鼠及蝙蝠,无菌采集其脑组织样本,用逆转录聚合酶链反应(RT-PCR)和细胞培养分离法检测狂犬病毒。结果 本次调查共捕获159只褐家鼠、66只蝙蝠(57只犬蝠、9只伏翼蝠),共获225份脑组织标本,RT-PCR和细胞培养分离法未在采集的脑组织样本中检测出狂犬病毒。结论 广州市褐家鼠、犬蝠及伏翼蝠作为狂犬病传染源的可能性较小。
Objective To examine the prevalence of rabies virus infection in commensal rodents and bats in Guang- zhou and to explore whether the rodents or bats are potential sources of human rabies virus infection. Methods Com- mensal rodents and bats were trapped in parks, hospitals, and markets in Guangzhou city between 2013 and 2015. Brain tissue samples of the trapped animals were collected under sterile. Rabies virus was detected by reverse transcription-poly- merase chain reaction (RT-PCR) and isolated with Vero cell culture. Results Totally 159 Rattus norvegicus and 66 bats (57 Cynopterus sphinx and 9 Pipistrelle) were trapped and 225 brain tissue samples were collected. No detectable rabies virus was found in the brain tissue samples either by RT-PCR or Veto cell culture isolation. Conclusion There is a low possibility that Rattus norvegicus, Cynopterus sphinx and Pipistrelle in Guangzhou could be infectious sources of human rabies virus infection.