中文摘要：

目的 探讨p38 MAPK抑制剂SB203580对软骨细胞NF-κB蛋白表达的影响。方法 体外培养兔关节软骨细胞,经甲苯胺兰染色鉴定后,一氧化氮（NO）供体NOC-18和p38 MAPK抑制剂SB203580作用于细胞24 h,用MTT比色法检测细胞增殖活性,Western blot测定NF-κB p65亚单位蛋白表达水平。结果 与对照组比较,NOC-18能抑制软骨细胞MTT增殖活性（P〈0.05）;NOC-18诱导的NF-κB p65活性的升高被SB203580抑制（P〈0.05）,而仅有SB203580作用于细胞时,与对照组比较,差别无统计学意义（P〉0.05）。结论 p38 MAPK促进了NO诱导的兔关节软骨细胞NF-κB的表达,NO致软骨细胞凋亡的信号通路中涉及了NF-κB的活性表达。

英文摘要：

Objective To explore the effect of p38 MAPK inhibitor SB203580 on chondrocyte protein expression of NF-κB. Methods Rabbit articular chondrocytes were cultured in vitro, and identified using toluidine blue staining. Following treatment with nitric oxide （NO） donor NOC-18 and p38 MAPK inhibitor SB203580 for 24 h, we performed MTT assay to evaluate the proliferation of cells, and Western blotting to determine the protein expression of NF-κB p65. Results NOC-18 significantly reduced chondrocyte proliferation, compared with untreated controls （P〈0.05）; SB203580 was able to suppress the accelerated activity of NF-κB p65 induced by NOC-18 （P〈0.05）. However, there was no significant difference when treated only with SB203580, compared with the control （P 〉 0. 05）. Conclusion The p38 MAPK promotes NO-induced rabbit articular chondrocyte NF-κB expression, and the increase of NF-κB expression is involved in NO-induced chondrocyte apoptosis pathway.