中文摘要：

目的：探讨热休克因子1（heat shock factor1，HSF1）对热应激所致Raw264．7巨噬细胞凋亡的影响。方法：采用热应激（42．5℃±0．5℃）处理稳定表达小鼠HSF1基因的Raw264．7巨噬细胞1h，37℃分别恢复6，9，12，24h，采用流式细胞术，hoechst33258染色和DNA琼脂糖凝胶电泳检测细胞凋亡。结果：流式细胞术结果显示，热应激后对照组（转空载体）细胞凋亡核百分率较热应激前明显升高，9h达峰值（约为60％），此时荧光染色可见30％的细胞出现核固缩，凋亡小体等典型的凋亡形态学改变；并于热应激后6，9，12h均能检测到清晰的DNA梯状条带。与转空载体对照组相比，HSF1过表达能显著降低热应激所致凋亡及明显抑制DNA的断裂。结论：HSF1可以抑制热应激所致的Raw264．7巨噬细胞凋亡。

英文摘要：

Objective To observe the effect of heat shock factor 1 （ HSF1 ） on heat stress-induced apoptosis in Raw264.7 macrophages. Methods Raw264.7 cells transfected with pcDNA3. 1 and pcDNA3. 1-HSF1 were exposed to heat stress （42.5 ℃ ±0.5 ℃ ） for 1 h and recovered at 37 ℃ for 6, 9, 12, and 24 h respectively. Flow cytometry （FCM） , Hoechst 33258 staining and DNA ladder assays were performed to assess the apoptosis. Results After heat stress, FCM showed that apoptotic cells were increased significantly and reached the peak at 9 h in Raw 264. 7 cells transfected with pcDNA3. 1 , and were characterized with classical morphologic changes including apoptotic body and nuclear condensation. Agarose gel electrophoresis showed that ＂ DNA ladder＂ could be observed clearly at 6, 9, and 12 h after the heat stress. But the overexpression of HSF1 could reduce the number of apoptotic cells and inhibit DNA fragmentation. Conclusion HSF1 can inhibit heat stress-induced apoptosis in Raw264.7 macrophages.