用AAPH热分解生成的自由基引发脂质氧化,通过硫氰酸铁法和硫代巴比妥酸反应物法检测脂质的氧化进程,研究欧李红色素在用大豆磷脂脂质体模拟非均相生物体系和用非离子表面活性剂Tween 20稳定的葵花油水包油乳状液模拟非均相食品体系中的抗氧化活性,同时考查其与VC或VE的协同作用。结果表明:在大豆磷脂脂质体中0.001 6、0.004、0.008 mg/m L和在葵花油乳状液中0.02、0.05、0.10 mg/m L的欧李红色素均具有抗氧化活性,且其抗氧化能力随浓度的升高逐渐降低。考察欧李红色素与VC或VE的协同作用时发现,在大豆磷脂脂质体中0.001 6 mg/m L VC或VE以及在葵花油乳状液中0.02 mg/m L VC或VE的加入,均使复合抗氧化剂不显示协同增效作用。
The antioxidant activity of the red pigment was investigated in soybean phosphatidylcholine liposome membrane and sunflower oil-in-water emulsion system exposed to AAPH generated peroxyl radicals.Total antioxidant activity of the crude extract was evaluated by measurement of the ferric thiocyanate assay and thiobarbituric acid test.The results indicated that the red pigment extracted from Cerasus humilis with the concentration of 0.0016 mg/mL,0.004 mg/mL,0.008 mg/mL in soybean phosphatidylcholine liposome membrane and the concentration of 0.02 mg/mL,0.05 mg/mL,0.10 mg/mL in sunflower oil-in-water emulsion system was a chain-breaking antioxidant.It was clear that antioxidant activities of individual the red pigment extracted from Cerasus humilis decreased with the rise of their concentrations.Furthermore,synergistic interactions between the red pigment extracted from Cerasus humilis and ascorbic acid/α-tocopherol against lipid peroxidation were also investigated.It was shown that the synergistic interactions were not found in the whole experiment.