中文摘要：

介绍了重构纳米类脂生物膜（r-NLM）的制备，并采用循环伏安法（CV）研究了温度和外加电场对r-NLM膜的影响，采用“相变温度”及“微桥”模型分析了其原因．从离子载体、离子通道的角度，分析了重构纳米类脂生物膜对不同离子的敏感机理．重构纳米类脂生物膜中离子载体型修饰剂，其不同大小的极性空腔能够络合不同半径的离子，并将离子运输到膜的另一边释放，从而实现对离子的传递；而离子通道型修饰剂则可在膜中形成不同直径的跨膜通道，通道开启时，可进行特定离子的选择性跨膜通道传输．

英文摘要：

A novel fabrication method of Reconstitution Nano-Lipid Biomembranes （r-NLM） was presented. Influences of temperature and applied voltage on r-NLM were investigated using Cyclic Voltammetry （CV）. Utilized ＂Phase Transfer Temperature＂ and ＂Micro-Bridge＂ model analyzed reasons of experimental results. Also, the sensing mechanism of r-NLM was analyzed according to ionic carrier and ionic channel perspectives. For the modifier of ionic carrier type embedded in r-NLM, the polarity cavity, which has different size, can chelated different radial ions. It can carry ions to the other side of modified membrane and release them. By this way, the ionic carrier performs the transportation of ions. On the other hand, the modifier of ionic channel type can form a transmembrane channel with different size in r-NLM. Some specification size ions can be transit across the r-NLM through the opened ionic channel.