中文摘要：

以5,5＇-二硫代双（琥珀酰亚氨基-2-硝基苯甲酸）（DSNB）分子作为偶联剂将蛋白质结合在金纳米粒子表面,既保持了蛋白质的生物活性,同时DSNB分子又具有较高的表面增强拉曼散射（SERS）活性,可作为蛋白质定量分析的探针分子.选用生物素与亲和素的特异性识别以及抗原抗体的免疫识别2个生物反应体系,将SERS纳米探针固定在蛋白质检测芯片上.以硅片为蛋白质检测载体,利用硅片在520 cm^-1处的拉曼特征峰为内标,对人IgG抗体进行定量分析.结果表明,该探针对人IgG抗体检测的最低浓度可以达到5 pg/mL.

英文摘要：

A biofunctionalized nanoprobe for surface-enhanced Raman scattering（ SERS）-based immunoassay was designed. 5,5＇-Dithiobis（ succinimidyl-2-nitrobenzoate）（ DSNB） was employed as a linker for protein and gold nanoparticle,which can help to maintain the biological activity of the proteins. Besides that,DSNB possesses a high Raman scattering section,which can be used as the SERS probe as quantitative determination of proteins. Two kinds of biological systems（ avidin-streptavidin and antigen-antibody） were selected for the target protein and nanoprobe specific binding to the protein chip. Meanwhile,silicon slide was employed as a carrier,which provides a Raman peak at 520 cm^-1 and can be used as a good internal standard for quantitative analysis of proteins. Using this method,anti-human IgG concentration in solutions can be quantitatively measured at concentrations as low as 5 pg / mL.