中文摘要：

目的检测并比较Rv1789刺激结核分枝杆菌（Mtb）感染人群和健康对照者外周血IFN-γ水平。方法以原核表达体系构建、表达、纯化rRv1789。从南京市疾病预防控制中心选择39位受试者,参考临床结核病诊断标准和rCM-WBIA诊断标准将其分为TB感染组（包括结核病患者和潜伏感染者）和健康对照组,进行rRv1789-WBIA试验。结果成功构建pET30b-Rv1789质粒,SDS-PAGE鉴定表达产物分子质量单位为44ku,与预期相符;Western blot证实该蛋白能被相应抗体识别。rRv1789刺激Mtb感染组产生的IFN-γ水平显著高于健康对照组（P〈0.01）;rCM和rRv1789刺激受检者全血IFN-γ水平具有显著相关性（r=0.616,P〈0.01）。结论 PE/PPE家族蛋白Rv1789具有抗原性,可用作Mtb感染快速诊断和结核疫苗候选抗原。

英文摘要：

Objectives To detect and compare levels of IFN-γstimulated by rRv1789 in peripheral blood from Chinese patients with tuberculosis（TB）and healthy controls. Methods rRv1789 was constructed,expressed,and purified using an Escherichia coli expression system.A total of 39 subjects selected by the Nanjing CDC were divided into subjects with TB（including patients with TB and individuals with a latent tuberculosis infection）and healthy controls based on criteria for clinical diagnosis of TB and a whole-blood IFN-γrelease assay based on the recombinant fusion protein CFP21-MPT64（rCM-WBIA）.Subjects were also tested using a WBIA based on the recombinant antigen Rv1789（rRv1789-WBIA）. Results pET30b-Rv1789 was successfully constructed and identified as correct.According to the results of SDS-PAGE,pET30b-Rv1789 had a molecular weight of about 44 ku,which agreed with the expected molecular weight.pET30b-Rv1789 was recognized by anti-His-tagged McAb according to Western blotting.The mean level of IFN-γdetected with the rRv1789-WBIA in patients infected with TB was statistically much higher than that in healthy controls（P=0.0002）.Moreover,the results of the rRv1789-WBIA were significantly correlated with those of the rCM-WBIA according to correlation analysis（r=0.616,P〈0.01）. Conclusion Results indicated the antigenicity of rRv1789 from the PE/PPE family,so Rv1789 might be a promising antigen for rapid diagnosis of TB and research on potential vaccines for TB.