中文摘要：

为分析泥河湾盆地大长梁遗址和马圈沟Ⅰ文化层中可培养放线菌的物种多样性,本实验通过平板稀释涂布法对该区域土样进行放线菌的物种分离,得到197株放线菌纯培养物。采用链霉菌属特异引物对放线菌纯培养物进行初步鉴定,确定143株放线菌归属于链霉菌属。对未被鉴定的54株放线菌进行16S rDNA全序列测定并分析其系统发育关系。最终,将197株放线菌归属于放线菌纲下的5个亚目,6个科,10个属。其中,马圈沟Ⅰ文化层的分离菌株12-9与Nocardioides albus KCTC 9186T之间的16S rDNA序列相似性是93.73%,可能是一个潜在新属;大长梁遗址的分离菌株18-64与Haloglycomyces albus YIM 92370T的16S rDNA序列相似性是93.64%,可能是一个潜在新属。

英文摘要：

In order t o analyze the species diversity of cultivable actinomycetes isolated from Dachangliang ancient site and Majuangou Ⅰ cultural stratum in Nihewan Basin, soil samples in this area were under species isolation of actinomycetes by dilution plate method, to obtain 197 strains of actinomycetes in pure culture. These pure cultures of actinomyces were preliminarily identified by the specific primers of Streptomyces and the results showed that 143 strains of actinomycetes belong to Streptomyces. 16S rDNA sequence determination was used for the phylogenetic analysis of the remaining 54 unidentified strains. Finally, the 197 strains of actinomycetes belong to 5 suborders, 6 families and 10 genera. Among these strains, the 16S rDNA sequence similarity of the strain 12-9isolated from Majuangou Ⅰ cultural stratum and the strain Nocardioides albus KCTC 9186 T is 93.73%, which suggests that it may be a potential new genus. The 16S rDNA sequence similarity of the strain 18-64 isolated from Dachangliang ancient site and the strain Haloglycomyces albus YIM 92370 Tis 93.64%, which suggests that it may belong to a potential new genus.