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外源基因导入对棉花烟粉虱种群的影响
  • ISSN号:2095-1353
  • 期刊名称:《应用昆虫学报》
  • 时间:0
  • 分类:S961.6[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]扬州大学园艺与植物保护学院,扬州225009, [2]扬州大学江苏省作物遗传生理重点实验室,扬州225009, [3]江苏里下河地区农业科学研究所,扬州225007
  • 相关基金:资助项目:转基因生物安全监测技术(2011ZX08012-004)、国家自然科学基金(30771272)和江苏省自然科学基金(07KJB210134).
中文摘要:

以转基因棉花和对应的常规棉花亲本为材料,探讨外源基因导入对棉花烟粉虱Bemisiatabaci(Gennadius)种群的影响。结果表明,在转基因棉花GK12、33B、SGK上,烟粉虱从卵发育到成虫羽化的历期分别为15.56、15.35和15.25d,较对应的常规棉亲本SM3(19.58d)、33(20.81d)、SY321(18.76d)分别短24.55%、26.23%、18.71%;GK12和33B棉花上烟粉虱的存活率(69.16%)分别较对应的常规棉亲本SM3(54.76%)和33(64.91%)高26.29%和12.81%,而SGK(63.21%)上烟粉虱的存活率与对应的常规棉亲本SY321(62.61%)之间差异不显著;烟粉虱在GK12(84.00)和33B(77.25)上的产卵量分别较SM3(62.25)、33(70.00)高34.93%和10.35%,但SGK和SY321之间差异不显著;在转基因棉花GK12、35B、SGK上,烟粉虱的雌雄性比分别比对应的常规棉亲本高26.71%、46.23%和19.17%。结果表明,外源基因导入后,有助于烟粉虱的发育,提高了烟粉虱的产卵量和雌雄性比,从而促进了烟粉虱种群的上升。

英文摘要:

The effects of expression of foreign insect-resistant genes in cotton plants on Bemisia tabaci (Gennadius) were studied. Developmental durations(from egg to adult)of B. tabaci on three different varieties of transgenic Bt cotton plants, GK12, 33B and SGK, were 15.56, 15.35, and 15.25 d, respectively. These were respectively 24.55% , 26.23% and 18.71% shorter than those on the conventional parental cotton varieties SM3,33 and SY321. The survival rates of B. tabaci on the transgenic Bt cotton varieties GK12 and 33B were 69.16% and 73.23% respectively, which were 26.29% and 12.81% higher than those on the parental varieties of these plants SM3 and 33 (54.76% and 64.91% ). There was no significant difference in the survival rate of B. tabaci on the transgenic Bt cotton variety GK12 (63.21%) and its parental variety SY321 (62.61%). The fecundity of B. tabaci on the transgenic Bt cotton varieties GK12 and 33B was 84.00% and 77.25% , which was 34.93% and 10.35% higher than that on the parental varieties SM3 (62.25%) and 33 (70.00%). There was no significant difference in the fecundity of B. tabaci on SGK and SY321. The percentage of female B. tabaci on the transgenic Bt cotton plants GK12, 33B and SGK was 26.71% , 46.23% and 19. 17% higher, respectively, to that on the parental varieties SM3, 33 and SGK. These resuhs indicate that the introduction of the foreign Bt gene promoted the development of B. tabaci and increased oviposition and the percentage of females of this pest on cotton plants.

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期刊信息
  • 《应用昆虫学报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国昆虫学会 中国科学院动物研究所
  • 主编:戈峰
  • 地址:北京朝阳区北辰西路1号院5号中科院动物所
  • 邮编:100101
  • 邮箱:entom@ioz.ac.cn
  • 电话:010-64807137
  • 国际标准刊号:ISSN:2095-1353
  • 国内统一刊号:ISSN:11-6020/Q
  • 邮发代号:2-151
  • 获奖情况:
  • 96、2000年获中科院《优秀期刊三等奖》,92年获中国科协《优秀学术期刊一等奖》,2001进入“中国期刊”方阵,“双百”期刊,排名第96位
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:3170