中文摘要：

目的探讨核因子-κB（NF-κB）靶向性寡核苷酸（oligodeoxynucleotides,ODN）“圈套”策略对创伤性炎症大鼠肝脏炎症介质IL-6的影响.方法Wistar大鼠96只,随机分成生理盐水对照组、创伤性炎症组、“圈套”ODN组和变异“圈套”ODN组.运用凝胶阻滞实验检测创伤性炎症术后肝脏组织NF-κB的活性及合成“圈套”ODN的体外竞争抑制,用RT-PCR和ELISA法检测大鼠肝脏组织IL-6的mRNA水平和蛋白水平.结果大鼠创伤性炎症术后3 h肝脏NF-κB的活性开始升高,在术后12 h达高峰.肝脏组织IL-6 mRNA水平和蛋白水平也明显上升,与肝脏NF-κB的活性改变一致,“圈套”ODN在体外能有效地抑制NF-κB活性.“圈套”ODN治疗后大鼠肝脏组织IL-6的mRNA水平和蛋白水平均明显下降,肝脏功能明显好转,而变异“圈套”ODN却没有效果.结论NF-κB“圈套”策略通过特异性抑制NF-κB活性,可以有效地抑制创伤性炎症大鼠肝脏炎症介质IL-6的释放.

英文摘要：

Objective To study the effects of decoy strategy targeted to NF-κB on IL-6 in rat＇s liver after traumatic inflammatin. Methods Ninty six Wistar rats were randomly divided into 4 groups： control group ,traumatic inflammation group, decoy ODN group, and mutant decoy ODN group. Rats were killed on 3, 6, 12, 24, 48, and 72 h respectively, for the determination of plasma ALT. Hepatocytes were isolated and nuclear protein was extracted, DNA binding activity of NF-κB was measured by EMSA. Decoy ODN＇s competition inhibition effect was assayed by EMSA. IL-6 gene expression in liver tissue was assessed by RT-PCR and IL-6 protein level was determined by ELISA. Results After traumatic inflammation, DNA binding activity of NF-κB in the liver increased. IL-6 mRNA and protein level also significantly increased and was in positive correlation with the activity of NF-κB. Decoy ODN effectively inhibited the activity of NF-κB ex vivo. After using decoy ODN, IL-6 mRNA and protein levels of liver tissue significantly decreased, plasma ALT levels were also significantly decreased. Conclusions Decoy strategy targeted to NF-κB could effectively inhibit rat＇s liver IL-6 release by inhibiting specifically the activity of NF-κB.