中文摘要：

目的：建立四逆泡腾片中甘草黄酮的高效液相色谱定量方法。方法：采用四逆泡腾片提取物酸水解方法制备甘草黄酮主要苷元一甘草素与异甘草素，采用HypersilODS2C18（4．6mm×250mm，5μm）色谱柱，以乙腈-水为流动相，梯度洗脱，流速0．8mL·min^-1，检测波长230nm，柱温40℃。结果：甘草素、异甘草素线性范围分别为0．0219—0．656txg、0．0216～0．647μg，r均为0．9999；平均加样回收率以甘草素与异甘草素的量之和计为100．6％，RSD为1．5％（n=6）。结论：该方法专属，分离效果好，灵敏度高，可用于四逆泡腾片的质量控制。

英文摘要：

Objective：To establish an HPLC for determination of licorice flavonoids in Sini effervescent tablets Methods ： An HPLC method was applied to separate and qualify the main components of licorice flavonoids,liquiriti- genin and isoliquiritigenin after acid hydrolysis was performed on these extracts. The samples were separated by a Hypersil ODS2 Cls column（4. 6 mm ×250mm,5μm） using acetouitrile -water as the mobile phase for gradient e- lution at a flow rate of 0. 8 mL · min-l,detection wavelength 230 nm,the column temperature 40 ℃. Results：The calibration curves were linear in the range of 0. 0219 - 0. 656 μg （ r = 0. 9999） for liquiritigenin, and 0. 0216 - 0. 647 g（ r = 0. 9999 ） for isoliquiritigenin; The sum of average recovery for liquiritigenin and isoliquiritigenin was 100.6%, and RSD was 1.5% （ n = 6）. Conclusion：The HPLC method developed for simultaneous determination of liquiritigenin and isoliquiritigenin is specific with good separation effect and high sensitivity which can be used for the quality control of Sini effervescent tablets.