中文摘要：

客观：与高分辨率建立二维的电气泳动侧面；从人的肺的重制度有鳞的癌织物；配对的正常肿瘤邻近的支气管的上皮，；识别微分表示由使用 proteome 分析的联系肿瘤的蛋白质。方法：有 20 人的肺的比较 proteome 分析有鳞的癌纸巾；邻近肿瘤的配对的正常支气管的上皮被执行。人的肺的全部的蛋白质有鳞的癌织物；配对的正常肿瘤邻近的支气管的上皮借助于使不能调动的 pH 被分开基于坡度的二维的胶化电气泳动(2-DE ) ；染色的银。微分表示蛋白质被分析；然后到飞行团分光术(MALDI-TOF-MS ) 的帮助矩阵的激光解吸附作用 / 电离时间识别了。结果：(1 ) 人的肺的解决得好的、可再现的 2-DE 模式有鳞的癌；邻近的正常支气管的上皮被获得。为肿瘤织物， 3 胶化的平均的点是 1567 ± 4 6，；4 看到的 1436 ± 5 与 91.6% 的平均匹配率被匹配。为控制， 3 胶化的平均的点是 1349 ± 5 8，；5 看到的 1228 ± 3 与 91.03% 的平均匹配率被匹配。匹配的点的平均位置偏差是在 IEF 的 0.924 ± 0 .128 公里方向，；在 SDS 页方向的 1.022 ± 0 .205 公里；(2 ) 6 看到的 1178 ± 5 的一个总数在 20 人的肺的电泳地图之间被匹配有鳞的癌纸巾；配对正常肿瘤邻近的支气管的上皮。76 差别表示了蛋白质被屏蔽；(3 ) 68 微分蛋白质被 PMF 识别，一些蛋白质是 oncogenes 的产品，；涉及房间的规定的其它骑车；信号转导变异；(4 ) 以便验证识别结果的可靠性， 3 蛋白质 mdm2 的表示， c-jun；EGFR，它与肺被相关有鳞的癌，被免疫检测组织化学的染色；西方的污点分析。结果揭示了那 mdm2， c-jun；EGFR 在肺是起来调整的有鳞的癌而他们在邻近的正常支气管的上皮是下面调整的，正常的肺纸巾；煽动性的假肿瘤，它与我们的 proteome 分析一致结果。结论：人的肺的解决得好的、可再现的 2-DE 模式有鳞的癌?

英文摘要：

Objective： To establish the two-dimensional electrophoresis profiles with high resolution and reproducibility from human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue, and to identify differential expression tumor-associated proteins by using proteome analysis. Methods： Comparative proteome analysis with 20 human lung squamous carcinoma tissues and the paired normal bronchial epithelial tissues adjacent to tumors was carried out. The total proteins of human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis （2-DE） and silver staining. The differential expression proteins were analyzed and then identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results： （1） Well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were obtained. For tumor tissue, average spots of 3 gels were 1567±46, and 1436±54 spots were matched with an average matching rate of 91.6%. For control, average spots of 3 gels were 1349±58, and 1228±35 spots were matched with an average matching rate of 91.03%. The average position deviation of matched spots was 0.924±0.128 mm in IEF direction, and 1.022±0.205 mm in SDS-PAGE direction; （2） A total of 1178±56 spots were matched between the eleetrophoretie maps of 20 human lung squamous carcinoma tissues and paired normal tumor-adjacent bronchial epithelial tissues. Seventy-six differentially expressed proteins were screened; （3） Sixty-eight differential proteins were identified by PMF, some proteins were the products of oneogenes, and others involved in the regulation of cell cycle and signal transduetion; （4） In order to validate the reliability of the identified results, the expression of 3 proteins mdm2, c-jun and EGFR, which was correlated with lung squamous carcino