中文摘要：

瞄准：调查方面人口(SP ) 的坚持在在药抵抗的胰腺的癌症和他们的角色和机制的房间。方法：当有教养时，在胰腺的癌症房间线 PANC-1 和它的比例的方面人口房间的演讲随着 Gemcitabine 变化，被 Hoechst 33342 染色和 FACS 分析检测。ABCB1 和 ABCG2 的表示被即时 PCR 在 SP 房间或 non-SP 房间检测。结果：SP 房间确实在 PANC-1 存在，与一 3.3% 的 median 和 2.1-8.7% 的一个范围。在以后与为 3 d 的 Gemcitabine 有教养， SP 房间的比例显著地增加了(3.8%+/-1.9% ， 10.7%+/-3.7% ， t = 4.616， P = 0.001 【 0.05 ) 。在 SP 在更高的集中表示为与 non-SP 房间相比的 ABCB1 和 ABCG2 (ABCB1：1.15 +/- 0.72， 5.82 +/- 1.16， t = 10.839， P = 0.000 【 0.05；ABCG2：1.16 +/- 0.75， 5.48 +/- 0.94， t = 11.305， P = 0.000 【 0.05 ) ，它可以贡献荧光灯的染色和药抵抗的流出。结论：有到化学疗法的代理人的内在地高的抵抗的 SP 房间确实在胰腺的癌症存在，它可以是贡献肿瘤的恶化的候选人癌症干细胞。

英文摘要：

AIM： To investigate the persistence of side population （SP） cells in pancreatic cancer and their role and mechanism in the drug resistance. METHODS： The presentation of side population cells in pancreatic cancer cell line PANC-1 and its proportion change when cultured with Gemcitabine, was detected by Hoechst 33342 staining and FACS analysis. The expression of ABCB1 and ABCG2 was detected by real- time PCR in either SP cells or non-SP cells. RESULTS： SP cells do exist in PANC-1, with a median of 3.3% and a range of 2.1-8.7%. After cultured with Gemcitabine for 3 d, the proportion of SP cells increased significantly （3.8% ± 1.9%, 10.7% ± 3.7%, t = 4.616, P = 0.001 〈 0.05）. ABCB1 and ABCG2 expressed at higher concentrations in SP as compared with non-SP cells （ABCB1： 1.15 ± 0.72, 5.82 ± 1.16, t = 10.839, P = 0.000 〈 0.05; ABCG2： 1.16 ± 0.75, 5.48 ± 0.94, t = 11.305, P = 0.000 〈 0.05）, which may contribute to the efflux of fluorescent staining and drug resistance. CONCLUSION： SP cells with inherently high resistance to chemotherapeutic agents do exist in pancreatic cancers, which may be candidate cancer stem cells contributing to the relapse of the tumor.