中文摘要：

目的：研究秀丽隐杆线虫（简称线虫）中一种扩展的Synaptotagmin同源物ESYT-2在致密核心囊泡分泌过程中起到的作用。方法：以线虫为研究对象,运用线虫腔胞吸收ANF-GFP的基本原理来确定ESYT-2与分泌相关,之后又进一步使用全内反射荧光显微镜技术（TIRFM）来研究ESYT-2对致密核心囊泡的具体调控。结果：①ESYT-2功能缺失影响线虫神经细胞致密核心囊泡分泌。②ESYT-2影响致密核心囊泡分泌的栓系过程。结论：ESYT-2调控了致密核心囊泡的分泌过程。

英文摘要：

Objective： To investigate the effect of ESYT-2 protein on DCV exocytosis in C.elegans.Methods： C.elegans was used as a model,neuropeptide release was assessed by measuring the fluorescence intensity of ANF-GFP in coelomocytes in vivo.Then the movements of single vesicles was tracked by using optical measurements（TIRFM）.Results： ① ESYT-2 loss of function animals exhibited a defect in DCV release.② ESYT-2 regulated the tethering step of DCV exocytosis in C.elegans.Conclusion： ESYT-2 affected DCV exocytosis in C.elegans.