中文摘要：

目的初步探讨整合素β1基因在RAW264.7细胞摄脂过程中的作用。方法针对整合素β1基因的不同靶点设计并化学合成3条具有阳性转染率的siRNA,用脂质体法转染RAW264.7细胞,并根据转染的方式将实验分为空白对照组、转染siRNA阴性对照组、脂质体对照组和转染筛选出的阳性转染率最高的siRNA组。用Cy3标记siRNA荧光显微镜下检测转染效率,Real-time PCR筛选沉默效率最高的siRNA,细胞黏附性实验检测细胞黏附性的改变,脂质油红O化学染色法检测细胞的脂质摄取量的变化,上述检测均重复测定3次。结果成功将siRNA转染入RAW264.7细胞,并成功筛选出沉默效率最高（65%）的siRNA为siRNA1,转染后实验组整合素β1mRNA表达明显低于其他组,细胞的黏附性从其他组的70%以上降低到53%,泡沫细胞转化率由其他组的90%以上显著降低到17%,摄脂能力显著降低,与其他组比较,差异有统计学意义（P〈0.05）。结论整合素β1基因明显影响RAW264.7细胞的摄脂功能,进而影响RAW264.7细胞向泡沫细胞的转化。

英文摘要：

Objective To investigate the effect of integrinβ1 gene on uptaking ox-LDL in RAW264.7 cells.Methods Three siRNAs were designed and synthesized according to different targets on integrinβ1 gene,and then transfected into RAW264.7 cells by lipidosome.RAW264.7 cells were divided into control（without transfection）,siRNANC（with transfection of siRNA negative）,lipo2000（without transfection but added lipo2000）,siRNA（with transfection of effective siRNA）.siRNA labeled by Cy3 was used for transfection efficiency detection,and real-time PCR was used for siRNA with highest silence efficiency screening.The change of cell adhesion was analyzed by cell adhesion experiment,and cell uptaking ox-LDL was evaluated by formation of lipid granules in the cells with oil red staining.Results siRNA integrinβ1 was successfully transfected into RAW264.7 cells with a highest silence efficiency of 65%.The expression of integrinβ1 mRNA was obviously decreased in siRNA1 group,in which cell adhesion was decrease form 70% to 53% as well as the foam cell formation was decreased from 90% to 17%.Statistical analysis showed the siRNA1 group had significant difference in ox-LDL uptake compared with the other groups（P0.05）.Conclusion Silencing integrinβ1 gene decreases the function of uptaking ox-LDL in RAW264.7 cells,and then inhibits the cells to transform into foam cells.