中文摘要：

调查草鲤鱼 preadipocytes 的区别机制，一个主要 adipocytes 文化系统被建立。汇合的 preadipocytes 被导致到区别，并且形态学和基因表达式在不同阶段被计算。preadipocytes 逐渐地充满微滴和在区别期间增加的细胞的类脂化合物内容，这被显示出。超微结构观察显示线粒体的数字与 adipocytes 区别增加了。一致地， mitochondrial 蛋白质内容在区分的 adipocytes 被提高。qRT-PCR 证明象 peroxisome proliferator 使活跃之物受体(PPAR ) 那样的 lipogenesis 相关的基因的表示水平，脂蛋白脂肪分解酵素(LPL ) ，丰满的酸 synthase (船边交货) 和 stearoyl-CoA desaturase (SCD ) 在 adipocytes 区别期间增加了。相关基因也提高了的 mitochondrial 什么时候 adipocyte 区别例如 PPAR coactivator-1 (PGC-1 ) ， PGC-1 和原子呼吸因素(NRF-1 ) 。然而，肉毒碱 palmitoyltransferase 的表示我(CPT-1 ) 基因在起始的阶段减少了，但是在房间区别的最后阶段增加了。这些结果显示草鲤鱼 preadipocytes 的区别过程类似于陆地动物的，但是分子的机制不是一模一样的。在这研究揭示的调查结果提供新信息给鱼 adipocyte 区别的学习。

英文摘要：

To investigate the differentiation mechanism of grass carp preadipocytes, a primary adipocytes culture system was established. Confluent preadipocytes were induced to differentiation, and the morphology and gene expression were evaluated at different stages. It was shown that preadipocytes were gradually filled with droplets and the cellular lipid content increased during the differentiation. Ultrastructure observation indicated that the number of mitochondria increased with adipocytes differentiation. Consistently, the mitochondrial protein content was ele- vated in the differentiating adipocytes, qRT-PCR showed that the expression level of lipogenesis-related genes such as peroxisome proliferator activator receptor 7 （PPAR 7）, lipoprotein lipase （LPL）, fatty acid synthase （FAS） and stearoyl-CoA desaturase （SCD） increased during adipocytes differentiation. The mitochondrial relevant gene also elevated when adipocyte differentiation, such as PPAR coactivator-1 （PGC-1 α）, PGC-1β and nuclear respiratory factor （NRF-1）. However, the expression of carnitine palmitoyltransferase 1α （CPT-1 α） gene decreased at the initial stage, but increased at the last stage of cell differ- entiation. These results indicated that the differentiation process of grass carp preadipocytes is similar to that of land animals, but the molecular mechanisms are not exactly the same. The findings revealed in this study provides new information to the study of fish adipocyte differentiation.