中文摘要：

背景 Resistin，最新发现的充满半胱氨酸的荷尔蒙主要由脂肪质的纸巾藏匿了，被建议了形成在肥胖和类型 2diabetes 之间的一个生物化学的连接。然而，在受体的 resist 还没被识别了。这研究瞄准了到 identifyresistin 绑定蛋白质 / 受体。三 cDNA 与一样碎裂的方法 11 bp 5'' 顺序被屏蔽老鼠的一个 cDNA 噬菌体显示图书馆发现多重纸巾。作为一样的读的框架， 11 bp 5'' 顺序被一条 TGA 站鳕鱼打断在上，匾电梯试金因而被用来证明读完现象。站鳕鱼在上在一样， 11 bp 5'' 顺序被色氨酸，和编码的肽的有约束力的活动代替[AWIL，它是被指明在有约束力的肽(RBP ) 抵抗] 与抵抗在被共焦的显微镜学技术和亲密关系层析实验识别。pDual GC-resistin 和 pDualGC-resistin 绑定肽是进 3T3-L1 房间的 co-transfected 证实功能 ofresistin 绑定肽。结果有一样的三 cDNA 碎片 11 bp 5'' 顺序被发现。TGA 站鳕鱼在上在读一样的框架， 11 bp 5'' 顺序被证明是 RBP 与的 readthroughed.The 绑定活动抵抗在因而被识别。在显著地表示 pDual GC-resistin 的 3T3-L1 preadipocytes 的 theresistin 绑定肽的表达式禁止了 adipogenic 区别。结论 RBP 能有效地救支持的区别在 overxepressed 3T3-L1 preadipocyte 抵抗。

英文摘要：

Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor. Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide. Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation. Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.