中文摘要：

hemin 有约束力的 DNA G 四倍(也作为 hemin aptamer 或 DNAzyme 知道) 以前被报导了能提高 hemin 的 peroxidase 活动。在这个工作，我们描述了有认出受动器的部分由二切开的 G 四倍的一半作为单个搁浅的 DNA 连接 flanked 出现的 DNAzyme 结构。在有完美地匹配的 DNA 海滨(受动器) 的酶的单个搁浅的部分的杂交形成了在二 G 四倍的一半之间双的僵硬 DNA 并且高效地因此压制了 G-quadruplex/hemin 建筑群的酶的活动，当错配的受动器海滨不能有效地调整 peroxidase 活动时。与 2,2-azinobis (3-ethylbenzthiazoline )-6-sulfonic 酸(ABTS ) 作为可氧化的底层，我们能描绘重新设计的 G-quadruplex/hemin 建筑群的形成并且验证它的可切换的 peroxidase 活动。我们的结果证明裂口 G 四倍是一个特别有用的模块向各种各样的生物学上或环境地有趣的目标设计便宜、没有标签的传感器。

英文摘要：

A heroin-binding DNA G-quadruplex （also known as a heroin aptamer or DNAzyme） has been previously re- ported to be able to enhance the peroxidase activity of heroin. In this work, we described a DNAzyme structure that had an effector-recognizing part appearing as a single stranded DNA linkage flanked by two split G-quadruplex halves. Hybridization of the single stranded part in the enzyme with a perfectly matched DNA strand （effector） formed a rigid DNA duplex between the two G-quadruplex halves and thus efficiently suppressed the enzymatic activity of the G-quadruplex/hemin complex, while the mismatched effector strand was not able to regulate the peroxidase activity effectively. With 2,2＇-azinobis（3-ethylbenzthiazoline）-6-sulfonic acid （ABTS） as an oxidizable substrate, we were able to characterize the formation of the re-engineered G-quadruplex/hemin complex and verify its switchable peroxidase activity. Our results show that the split G-quadruplex is an especially useful module to design low-cost and label-free sensors toward various biologically or environmentally interesting targets.