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中文摘要：
目的 应用非侵入性活体显像技术研究血管生成抑制因子vasostatin。方法 采用融合表达方案，将治疗基因vasostatin和报告基因fluc偶联构建融合表达载体，使表达的融合蛋白中两个蛋白互相不干扰，并有天然的特性。结果 将稳定表达FLuc阳性对照和V10FL融合蛋白的PC3细胞进行体外生物发光显像。用稳定表达FLuc的PC3细胞构建荷瘤模型，用生物发光显像能检测到肿瘤的发生。结论 可应用非侵入性活体显像技术进行体内和体外基因表达的检测与监控。
英文摘要：
Objective To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin. Methods We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character, Results We therefore examined clones of PC3 cells stably expressing fusion gene and positive control fluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks. Conclusion Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro,
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