依照OECD 235和218指导准则,选用溪流摇蚊(Chironomus riparius)幼虫为受试生物,以存活率、羽化时间为评价指标,通过暴露于加标水中(48 h)和加标沉积物中(28 d)两种方式研究菲对摇蚊幼虫的急、慢性毒性。结果表明:对于急性毒性实验,在加标水中菲对摇蚊幼虫的LC50为1.1 mg·L^-1(24 h)和337.2μg·L^-1(48 h)。对于慢性毒性实验,在加标沉积物中菲对摇蚊幼虫的28 d-LC50为24.9 mg·kg^-1;孔隙水中菲对摇蚊幼虫的28 dLC50为10.4μg·L^-1;另外,随沉积物中菲浓度增加,幼虫的平均羽化时间提前,当沉积物中菲浓度达到5 mg·kg^-1及以上时,受试幼虫的平均羽化时间比对照组及低浓度组显著提前(P〈0.05),在菲浓度为10 mg·kg^-1时比对照组提前了3.8 d,而提前时间在大于5mg·kg^-1的各浓度处理之间无显著差异(3.7-4.2 d)。
According to the guideline of OECD 235 and 218, the toxicity of phenanthrene (Phe) to Chironomus riparius larvae was studied. In the toxicity experiments, survival rate and emergence time were used as endpoints. The acute toxicity (48 h) was assessed by using spiked water and the chronic toxicity (28 d) was assessed by using spiked sediments. The results showed that for acute toxicity, the 24 h and 48 h lethal concentrations to 50% of organisms (LC50) of Phe in water to C. ripurius larvae were 1100 and 337.2 μg·L-1, respectively. For chronic toxicity, the 28 dLC50 of Phe in sediment and in pore water to C. ripurius were 24.9 mg·kg^-1 and 10.4 μg·L-1, respectively. Additionally, the mean emergence time of the larvae was advanced with the increasing concentration of Phe in sediments. When the concentration of Phe in sediments increased to 5 mg·kg^-1, the average emergence time of test organisms was significantly earlier than control and lowexposure concentration groups (P〈0.05). When the exposed concentration in sediments increased to 10 mg·kg^-1, the average emergence time was 3.8 d shorter than control group, and from 5 mg·kg^-1 on, the average emergence time of different exposure concentrations was similar to each other (3.7-4.2 d).