中文摘要：

从大豆（Glycinemax）中克隆了一个与抗逆相关的DREB（Dehydration Responsive Element Binding Protein）基因GmDREB5。功能分析证明,GmDREB5基因能够显著提高烟草的抗旱性和耐盐性。为了筛选GmDREB5的互作蛋白,采用酵母双杂交系统以GmDREB5蛋白73～226位氨基酸区段为诱饵筛选干旱处理的大豆cDNA文库,发现一个互作蛋白含有保守的WD40结构域,与棉花（Gossypium hirsutum）和水稻（Oryza sativa）的G蛋白β亚基分别具有61%和52%的同源性,说明蛋白可能是一类新的大豆G蛋白β亚基,将其定名为GmGβ1。将GmDREB5与GmGβ1共转化酵母菌株AH109,转化的酵母能够在四营养缺陷型培养基上（SD/trp^-leu^-his^-ade^-）正常生长,而对照不能生长;同时,共转化的酵母能够激活LacZ报告基因的表达,证明GmGβ1与GmDREB5之间存在相互作用。表达特性分析表明,GmGβ1基因受干旱、低温、高盐等胁迫和激素ABA处理的诱导而表达,证明GmGβ1不仅参与植物对非生物胁迫的响应,同时参与对GmDREB5蛋白水平的调控。

英文摘要：

It is well know that the DREB （dehydration responsive element binding protein） transcription factors in the AP2/ERF family play important roles in the regulation of plant stress responses, hormone responses and so on. GmDREB5, a novel DREB homologous gene, was isolated from soybean （Glycine max） and transferred into tobacco in our previous study. To investigate its function, the T2 transgenic tobacco plants with GmDREB5 and the wild type plants were inoculated on MS medium with 100 mmol L-1 NaCl and 2% PEG and their phenotypes were observed 40 days later. The results showed that the growth of the transgenic plants was more vigorous, and their roots were longer on the medium containing 2% PEG than those of wild type plants. The germination rate of transgenic plants （75%） was five-fold higher than that of the wild type plants （15%） on the medium containing NaCl, indicating that the over-expression of GmDREB5 improved the tolerances to both drought and salt stresses. The interacting proteins of GmDREB5 was attained by screening the cDNA library of soybean under drought and identified by yeast-two-hybrid system. It was found that there existed conservative domain WD40 in the interacting proteins,and which shared 61% and 52% similarity of the amino acid sequence with G protein D subunit in cotton （Gossypium hirsutum） and rice （Oryza sativa）, respectively, The interacting protein with GmDREB5, named GmGβ1, was isolated and proven to be a G protein β subunit in soybean. The prey vector containing GmGβ1 and bait vector pGBKT7-IIR were co-transformed into yeast AH109, then incubated on mediums with shortage of four components （SD/trp^- leu^- his^- ade^-） for 3-5 days, and the results showed that the thans-formants but the contorol grew normally ,and could activate the expression of the downstream reporter gene LacZ, which performed interaction between GmDREB5 and GmGβ1. Further analyses showed that the expression of GmGβ1 gene in soybean was induced by drought, high salt, cold stresses and