中文摘要：

目的：分析重组大肠杆菌BL21（pET28a-eIF5A）的抗逆性。方法：将来源于柽柳的eIF5A基因构建到原核表达载体pEW28a中，在原核表达的基础上，利用分光光度计检测重组大肠杆菌BL21（pET28a-eIF5A）的抗逆性。结果：BL21（pET28a-eIF5A）的抗盐碱性明显高于对照菌株BL21（pEW28a），其中重组大肠杆菌BL21（pEW28a-eIFSA）的抗NaCl的临界浓度为0．8mol／L，抗NaHCO3的临界浓度为0．52mol／L，不具有抗AgNO3和抗旱性。结论：重组大肠杆菌BL21（pET28a-eIF5A）具有良好的抗NaCl和NaHCO3特性，该抗性的证明为eIF5A基因在植物抗逆基因工程中的应用提供了资料。

英文摘要：

Objectives： To get the fundamental fimetion of the Eukaryote transcription initiation factor 5A （eIF5A）. Methods： The gene encoding eIF5A was cloned from Tamarix chinensis and ligated to the prokaryotic expression vector pET28a, and transform the E. coli strain B121. Then, the recombinant was subject to salt and alkali stress resistance analysis based on its OD600 variation under different salt and alkali concentrations. Results： The results showed that the recombinant exhibit strong tolerance to salt and alkali than the non- recombinant bacteria, and the critical concentration of NaCl and NaHCO3 are 0.8mol/L and 0.52 mol/L, respectively ; but has not obvious resistant to AgNO3 and dry stress. Conclusion： E. coli BL21expressing the eIF5A gene had obvious resistance to salt and alkali stress, these provide preliminary information for the application of eIF5A gene in plant gene engineering.