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人卵巢癌高转移细胞株裸鼠原位移植瘤模型
  • ISSN号:1000-7857
  • 期刊名称:科技导报
  • 时间:0
  • 页码:32-35
  • 语言:中文
  • 分类:R445.1[医药卫生—影像医学与核医学;医药卫生—诊断学;医药卫生—临床医学]
  • 作者机构:[1]重庆医科大学生物医学工程系、重庆医科大学生物医学工程学重点实验室、重庆市超声医学工程重点实验室一省部共建国家重点实验室培育基地,重庆400016
  • 相关基金:国家自然科学基金项目资助(编号:10774198)
  • 相关项目:强度聚焦超声场中声敏剂、微气泡和抗肿瘤药物相互作用研究
作者: 于廷和|张骄|
中文摘要:

目的:通过与亚甲蓝(Methylene blue,MB)示踪法比较,探讨超声造影检测乳腺癌前哨淋巴结(Sentinel lymph node,SLN)的前景。方法:建立兔VX2乳腺癌模型24只,皮下注射SonoVue,超声造影显像SLN,观察其造影增强情况;再经皮下注射MB示踪;行腋窝淋巴结清扫术切除SLN并行AE1/AE3免疫组化染色。结果:24只瘤兔超声造影检出回声均匀性增强的SLN 12个,回声不均匀性增强和部分充盈缺损的SLN 22个;MB示踪法检出SLN 32个;术中腋窝淋巴结清扫SLN 38个。以手术切除为标准,超声造影的检出率(89.47%)与MB示踪法检出率(84.21%)比较无显著差异(P〉0.05);以免疫组化结果为标准,超声造影的敏感性(95.24%)与MB示踪法敏感性(91.30%)比较无显著差异(P〉0.05),但其特异性(84.62%)和准确性(91.18%)均明显高于MB示踪法特异性(26.67%)和准确性(65.79%)(P〈0.05)。结论:经皮下注射超声造影剂显像乳腺癌SLN有较高的检出率、特异性、准确性和敏感性,与MB示踪法比较有一定优势,有望成为检测SLN的常用方法。

英文摘要:

Objective:To explore the prospects of contrast-enhanced ultrasound and methylene blue(MB)detection of breast cancer sentinel lymph node(SLN).Methods:Twenty-four rabbit models of VX2 breast cancer was set up with,subcutaneous injection of SonoVue,to get contrast-enhanced ultrasound imaging of SLN.The situation of contrast-enhanced SLN was observed.Thenubcutaneous injection of MB was performed to trace SLN.SLN was resected from axillary lymph nods,which underwent immuno-histochemistry of AE1/AE3.Results:Among the twenty-four rabbits,12 SLN homogeneous echo-enhanced.22 SLN enhanced non-uniformity were found by contrast-enhanced ultrasound.By MB tracer method 32 SLN were detected.38 SLN were resected from axillary lymph nodes.To make surgical resection as the standard,the detection rate of contrast-enhanced ultrasound(89.47%) and the MB tracer detection rate(84.21%) showed no significant difference(P0.05).To make the results of immunohistochemistry criteria as the standard,contrast-enhanced ultrasound sensitivity(95.24%) and the MB tracer method sensitivity(91.30%) showed no significant difference(P 0.05),but the specificity(84.62%) and accuracy(91.18%) were significantly higher from contrast-enhanced ultrasound sensitivity than those from tracer MB method-specificity(26.67%) and accuracy(65.79%)(P0.05).Conclusion:Contrast-enhanced ultrasound imaging indetecting SLN of breast cancer gets a higher detection rate,specificity,accuracy and sensitivity compared with the MB tracer method,which is expected to become the common method in detection of SLN.

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期刊信息
  • 《科技导报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国科学技术协会
  • 主编:项昌乐
  • 地址:北京市海淀区学院南路86号科技导报社
  • 邮编:100081
  • 邮箱:kjdbbjb@cast.org.cn
  • 电话:010-62138113
  • 国际标准刊号:ISSN:1000-7857
  • 国内统一刊号:ISSN:11-1421/N
  • 邮发代号:2-872
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国乌利希期刊指南,美国剑桥科学文摘,英国科学文摘数据库,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:24858