以载片上培养的大肠杆菌生物膜为对象,研究了氯对模拟管壁生物膜中大肠杆菌的灭活效果,同时考察氯氧化生物膜后水中生物可同化有机碳(AOC)、生物可利用磷(MAP)和细菌生长潜能(BRP)的变化情况.结果表明,氯可以有效灭活悬浮态的大肠杆菌,而对生物膜中的大肠杆菌的灭活效率远低于悬浮菌;在相同CT值下,较高浓度的氯对悬浮态和生物膜中大肠杆菌的灭活效果要高于低浓度的氯.氯对生物膜的氧化作用会使生物膜中物质溶出,增加了水中AOC和MAP浓度,如当氯的浓度为1.0 mg/L(CT值为100 mg·min/L)时,水中AOC由20.78μg/L增加到120.17μg/L,MAP含量由0.11μg/L增加到0.17μg/L;氯的氧化作用会增加水的细菌生长潜能(BRP),BRP随着CT值的增加而增加,如当氯投量为1.0 mg/L(CT值为100 mg·min/L)时,BRP可达到1.10×10^7CFU/mL.
In the article, the inactivation effect of chlorine on E. coli biofilm and the influence of chlorine oxidization on the contents of assimilable organic carbon (AOC), microbially available phosphorus (MAP) and bacterial regrowth potential (BRP) was investigated in the simulated drinking water distribution system. Results showed that chlorine resulted in more efficient reduction on suspended E. coli than did in biofilm. The inactivation effect of E. coli was influenced by chlorine concentration. Likewise, higher chlorine concentration resulted in more E. coli inactivation rate at the same CT (chlorine concentration multiply by time) value, when biofilm was oxidized by chlorine. Concentrations of AOC and MAP in bulk water increased owing to organic substance dissolved from biofilm. The AOC concentration increased from 20.78 μg/L to 120.17 μg/L, and the MAP was increased from 0.11 μg/L to 0.17 μg/L, and the Chlorine oxidization enhanced BRP concentration in the bulk water. BRP reached maximum at 1.10 × 10^7 CFU/mL when chlorine concentration was 1.0 mg/L, CT value was 100 mg· min/L.