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复合菌剂提高果树枝条堆肥过程中酶活性
  • ISSN号:1002-6819
  • 期刊名称:农业工程学报
  • 时间:2015
  • 页码:244-251
  • 分类:X71[环境科学与工程—环境工程]
  • 作者机构:[1]西北农林科技大学资源环境学院,杨凌712100, [2]西北农林科技大学理学院,杨凌712100
  • 相关基金:国家自然科学基金(No.4087119,41171203);公益性行业(农业)科研专项(201303094);陕西省重大攻关项目(2011KTZB02.02).
  • 相关项目:养殖场废弃物中残留的抗生素对土壤微生物种群和酶活性的影响
中文摘要:

为了探讨接种复合菌剂对果树枝条堆肥过程的影响,以猪粪和果树枝条为试验材料,研究了复合菌剂对高温好氧堆肥过程的温度、酶活性及微生物群落功能多样性的影响。结果表明,接种复合菌剂在堆肥前期提高堆肥温度,比对照处理高温期(高于55℃)持续时间多3d,促进堆料的腐熟。接种菌剂还能有效地提高果树枝条堆肥过程中酶活性,纤维素酶、漆酶、锰过氧化物酶和木质素过氧化物酶活性在堆肥过程中分别比CK处理提高15.0%~19.8%、1.0%~11.0%、4.1%~26.8%、4.0%~22.2%。Biolog 微平板法测定结果表明,接种复合菌剂显著提高了堆肥中微生物的平均每孔颜色变化率(average well color development),并改变了高温期和降温期微生物对6大类碳源的利用;主成分分析表明,复合菌剂主要在高温期发挥作用,对堆料中微生物起分异作用的碳源主要为糖类和氨基酸类。

英文摘要:

The effects of compound microbial agents in compost with mixed fruit tree branches and pig manure during composting in a thermophilic aerobic state were examined .The research used the inoculants compound microbial agents and control compost. The changes of temperature, enzyme activity, and the microbial community functional diversity in compost materials during composting were determined. The results showed the temperature during the composting period was increased and the high temperature (above 55℃) maintaining period was three days longer. Besides, inoculation treatment and control treatment are all carried out to achieve compost maturity, what was more, when added to the compound microbial agents composting process, we can easily find that the number of the seed germination in inoculants treatment is obviously higher than it is in the control treatment (P<0.05), so we can find that this process caused the decrease of the pH value and the C/N ratio. Of course, they promoted the compost maturity. The activities of the enzymes were improved by adding compound microbial agents. The tested enzymes Cellulose, laccase (Lac), Manganese peroxidase (MnP), and lignin peroxidase (LiP) activities were higher in the treatment with microorganism agent than those in the control treatment by 15.0%-19.8%, 1.0%-11.0%, 4.1%-26.8%, and 4.0%-22.2%, respectively. The research showed that the activity of Cellulose, Laccase (Lac), and the lignin peroxidase (LiP) rose at the beginning of the experiment and then dropped gradually under the inoculants agent processing, and reached the peak value in the seventh day. Their numerical values were 0.96 mg/(g·d) and 72.6 U/g respectively. However, the Manganese peroxidase (MnP) and Lignin peroxidase (LiP) reached their peak in the second day. Their numerical peak values were 927.2 and 726.4 U/g respectively. In addition, the activity of Manganese peroxidase (MnP) which was dealt with the inoculation treatment and control treatment was

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期刊信息
  • 《农业工程学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国农业工程学会
  • 主编:朱明
  • 地址:北京朝阳区麦子店街41号
  • 邮编:100125
  • 邮箱:tcsae@tcsae.org
  • 电话:010-59197076 59197077 59197078
  • 国际标准刊号:ISSN:1002-6819
  • 国内统一刊号:ISSN:11-2047/S
  • 邮发代号:18-57
  • 获奖情况:
  • 百种中国杰出学术期刊,中国精品科技期刊,中国科协精品科技期刊工程项目期刊,RCCSE中国权威学术期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国工程索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:93231