中文摘要：

在环境的重金属是有害的为植物的正常生长和发展限制因素。这里，我们孤立并且识别 Arabidopsis thaliana T-DNA 插入说出变异的、 sro1-1，它显示出过分敏感的回答到 HgCl < 潜水艇 class= “ a-plus-plus ” > 2 。SRO1 蛋白质包含调停的一个 WWE 领域蛋白质蛋白质相互作用。在 HgCl 下面 < 潜水艇 class= “ a-plus-plus ” > 2 处理与野类型的植物相比， sro1-1 的生长戏剧性地被镇压，真叶子的数字被减少并且变白。电解质漏率证明在 sro1-1 的房间膜正直比在野类型更严重地被损坏。轻拍(3,5-diaminobenzidine ) 染色并且共焦的显微镜学显示出那 Hg < 啜 class= “ a-plus-plus ” >2+ 应力比在野类型在 sro1-1 导致了更多的氢过氧化物累积。qRT-PCR 结果显示一些不能生活的导致压力的基因的表示例如 L-ascorbate peroxidase (APX1 ) ，被减少在下面氧化或 Hg < 啜 class= “ a-plus-plus ” >2+ 应力。包含 GFP::SRO1 熔化蛋白质的转基因的植物证明 SRO1 在房间的原子核是局部性的。SRO1 被显示在各种各样的纸巾被表示，并且最高度在精力旺盛的纸巾被表示。我们的结果建议 SRO1 可以在 A 的压力反应起一个重要作用。thaliana 到重金属。

英文摘要：

Heavy metals in the environment are harmful limiting factors for the normal growth and development of plants. Here, we isolated and identified an Arabidopsis thaliana T-DNA insertion mutant, named srol-1, which showed a hyper-sensitive response to HgCl2. The SRO1 protein contains a WWE domain that mediates proteinprotein interactions. Under HgCl2 treatment, when compared with the wild-type plants, the growth of srol-1 was repressed dramatically and the number of true leaves was reduced and etiolated. The electrolyte leakage rates showed that cell membrane integrity in srol-1 was damaged more severely than in the wild type. DAB （3,5-diaminobenzidine） staining and confocal microscopy showed that Hg2＋ stress induced more hydrogen peroxide accumulation in srol-1 than in the wild type. The qRT-PCR results indicated that the expression of some abiotic stress-induced genes, such as L-ascorbate peroxidase （APX1）, was reduced under oxidative or Hg2＋ stress. Transgenic plants containing a GFP：：SRO1 fusion protein showed that SRO1 was localized in the nucleus of the cells. SRO1 was shown to be expressed in various tissues, and was most highly expressed in the vigorous tissues. Our results suggest thatSRO1 may play an important role in the stress response of A. thaliana to heavy metals.