中文摘要：

目的观察四物汤有效成分配伍组方对电离辐射所致人淋巴母细胞AHH-1细胞氧化损伤的辐射防护作用，并初步探讨其作用机制。方法4．0Gy 60Coγ射线单次照射AHH-1细胞，造成电离辐射损伤。观察不同浓度的四物汤有效成分配伍组方（果糖、阿魏酸、芍药苷、川芎嗪质量比为9008：53：721：267）对于电离辐射损伤的AHH-1细胞活力、凋亡情况、活性氧（ROS）水平的影响，以及对pGL4-ARE报告基因载体的激活作用。结果四物汤有效成分配伍组方在10mg／L的浓度下抗辐射效果最为显著，可以减缓受照AHH-1细胞活力下降（t=-8．152，P〈0．05），缓解早期细胞凋亡（t=12．777，P〈0．05），降低ROS水平（t=3．465，P〈0．05），诱导报告基因pGIA．ARE荧光表达倍数提高（t=-19．752，P〈0．05）。结论四物汤有效成分配伍组方具有体外抗辐射活性，可能通过激活Nrf2-Keap1—ARE抗氧化通路、降低辐射所致氧化损伤，从而发挥辐射防护作用。

英文摘要：

Objective To observe the effects of Siwu Tang formulation, which consists of fructose, ferulie acid, paeoniflorin, and ligustrazine hydrochloride （mass ratio of 9 008： 53： 721： 267）, on the oxidative damage in irradiated AHH-1 cells, and to investigate the underlying protective mechanism. Methods AHH-1 cells were irradiated with a single dose of 4.0 Gy S60Co γ-rays, then cell viability, apoptosis and reactive oxygen species （ROS） level were measured. The activation of Nrf2-Keapl-ARE pathway was observed by transferring pGL4-ARE reporter gene transiently into HEK293 ceils before the administration of Siwu Tang. Results At the concentration of 10 mg/L, Siwu Tang components formula had the best efficiency on anti-radiation damage. This drug inhibited the reduction of cell viability （t = - 8. 152, P 〈 0. 05 ）, mitigated apoptosis （ t = 12. 777, P 〈 0. 05 ）, reduced intracellular ROS levels （ t = 3. 465, P 〈 0. 05） , and induced the expression of pGIA-ARE fluorescence reporter gene （ t = - 19. 752, P 〈 0. 05 ）. Conclusions Siwu Tang formulation has a protective effect against radiation by reducing radiation-induced oxidative damage through the activation of the Nrf2-Keap1-ARE signaling pathway.