Superoxide dismutase (草皮, EC 1.15.1.1 ) 首先并且最对氧化应力的细胞的防卫的重要的线。在这学习,基于 P 的 unigene 序列。haitanensis,三全身的 PhSOD 基因被赛跑技术,和命名 PhMSD 获得, PhCSD1 和 PhCSD2。这些基因的全身的 cDNAs 包括了 973, 1 029 和 954 核苷酸分别地。cDNAs 分别地编码了 224, 134 和 216 氨基酸的蛋白质,与 5.75, 4.65 和 10.74 的等电位的点。基于他们的保存主题和种系发生的树分析,三 PhSODs 被划分成二种草皮类型:PhMSD 是一张 Mn 草皮,并且 PhCSD1 和 PhCSD2 是 CuZn 草皮。qPCR 被用来在 P 的不同生活阶段测量三 PhSOD 基因的表示。haitanensis,并且在高温度的应力的不同时期和干燥的不同层次下面。在 P 的不同生活阶段。haitanensis,三 PhSODs 的表示层次都比在 conchocelis 在叶状体是显著地更高的。在期间高温度并且干燥应力, PhCSD1 和 PhCSD2 的表示层次被 O 2- 内容,而是 PhMSD 的表示水平高度导致被高温度应力高度压抑并且没在干燥期间显示出重要变化。
Superoxide dismutase(SOD,EC 1.15.1.1) is the first and most important line of cellular defense against oxidative stress.In this study,based on unigene sequences of P.haitanensis,three full-length Ph SOD genes were obtained by RACE technology,and named Ph MSD,Ph CSD1 and Ph CSD2.The full-length c DNAs of these genes comprised973,1 029 and 954 nucleotides,respectively.The c DNAs encoded proteins of 224,134 and 216 amino acids,with isoelectric points of 5.75,4.65 and 10.74,respectively.Based on their conserved motifs and phylogenetic tree analysis,the three Ph SODs were divided into two SOD types:Ph MSD is a Mn-SOD,and Ph CSD1 and Ph CSD2 are Cu Zn-SODs.q PCR was used to measure the expression of the three Ph SOD genes in different life phases of P.haitanensis,and under different periods of high-temperature stress and different levels of desiccation.In the different life phases of P.haitanensis,the expression levels of the three Ph SODs were all significantly higher in the thallus than in the conchocelis.During high-temperature and desiccation stress,the expression levels of Ph CSD1 and Ph CSD2 were highly induced by the O2-content,but the expression level of Ph MSD was highly depressed by high temperature stress and showed no significant change during desiccation.