中文摘要：

为了研究Na Cl浓度、金属离子（Cr^7＋、Mg^2＋及K^＋）、氯化钪及卤素（I^-、Br^-及F^-）对嗜盐放线菌株YIM90615液体发酵过程中次级代谢产物多样性的影响,作者采用HPTLC（high performance thin layer chromatography）技术,结合紫外（254 nm）检测、碘及硫酸-茴香醛显色方法,对该菌代谢产物的HPTLC图谱进行了定性分析.结果显示,Na Cl添加质量浓度为100 g/L时该菌株发酵效果最佳,有利于其代谢产物的多样性及量的积累;Cr^7＋和K^＋有利于其次生代谢产物的产生;1 g/L的氯化钪能增加其次生代谢产物的丰富性;F-对其次生代谢产物多样性有较好的促进作用.试验结果表明外源添加一定浓度和种类的盐离子有助于提升YIM 90615液体发酵过程中次生代谢产物多样性,为新型抗生素及其它有用化合物的产生创造合适条件,同时有助于嗜盐放线菌发酵培养基的筛选工作.

英文摘要：

To understand the effect of salt irons, such as NaCl, metal irons （ Cr^7＋, Mg^2＋, and K^＋ ）, SeCl3, and halogen （I^-, Br^-, and F^-） ,on the diversity of secondary metabolites produced by Actinopolyspora YIM 90615, HPTLC chromatographed by UV254, iodine and anisaldehyde-sulfuric acid were performed.The results showed that application of NaC1 at the final concentration of 100 g/L showed the best promotion to the growth of the strain and the diversity of secondary metabolites production, and Cr^7＋ , K^＋ also help to produce more secondary metabo- lites, additional 1 g/L ScCl3 and F- could promote the diversity of secondary metabolites too.These results sugges- ted that exogenous application of salt irons could obviously stimulate the growth of strain YIM 90615 and the pro- ductivity and diversity of second metabolisms, which could provide the theoretical basis for the practical applica- tion of Actinopolyspora in future and create suitable conditions for producing new antibiotics and other useful com- pounds.It also provides theoretical basis for the selection of the fermentation medium for other Actinomycetes.