中文摘要：

通过PCR扩增，从拟南芥中克隆到长度为903bp的UGT71C5基因启动子，并构建了此种启动子与GUS嵌合的重组载体，通过农杆菌介导法转化拟南芥，得到转基因拟南芥，并且通过启动子分析软件对全序列进行分析，发现在该段序列中含有典型的TATA—box、CAAT—box和光应答元件GTl、干旱应答元件ERD等一些顺式作用元件．利用分光光度法测定转基因植株在光照、干旱和ABA等胁迫处理下的GUS酶活力，结果表明：转基因植株的GUS酶活力介于野生型和35S阳性对照之间，与正常条件下生长的转基因植物相比，光照处理8h和12h后其GUS酶活力分别增加3倍和4倍；干旱和ABA胁迫处理后并未发现酶活力右明昂酌增疆可刃．篡因r价7、71C5扈动平时弈．鳢右廊基．而对平旱南ARA开．府基

英文摘要：

A 903bp DNA fragment in regulation region of UGT71C5 gene from Arabidopsis thaliana was cloned by PCR method. To investigate the function of this promoter, full length DNA of UGT71C5 promoter was constructed into eukaryotic expression vector pBI121, and introduced into Arabidopsis plants （RLD） via Agro- bacterium-mediated transformation. The transgenic plants were selected on MS supplied with Kan and confirmed through PCR. The full length of this promoter was analyzed with some sequence analysis soft wares. The result showed that the promoter sequence included typical TATA-box, CAAT-box and some cis-acting elements which had relation to light-regulation of plant and drought response and so on. The GUS activity of the transgenic plants was analyzed by spectrophotometry; the result showed that the activity of the transgenic plants was found between the wild type and positive plants. The GUS activity of the transgenic plants was also determined under light, drought, and ABA stress, it was found that compared with the control the activity of the transgenic plants increased about fourfold and fivefold under light stress for 8h and 12h, and the activity didn＇t change under drought,and ABA stress.