中文摘要：

目的：建立并优化白念珠菌总蛋白质组分析的二维电泳条件。方法：以白念珠菌国际标准菌株SC5314为材料，制备其总蛋白质组样品。以真菌细胞破碎方法、裂解液成分、上样量、固相pH梯度胶条的选择为侧重点，通过不同条件下电泳图谱的比较建立最佳二维电泳技术条件。结果：成功建立白念珠菌总蛋白质制备方法，优化了白念珠菌蛋白质组分析的二维凝胶电泳技术，获得重复性和分辨率都较理想的白念珠菌总蛋白质二维电泳图谱。结论：优化后的二维电泳条件符合白念珠菌的特点，为白念珠菌蛋白质组分析奠定基础。

英文摘要：

Objective：To develop and optimize a two-dimensional（2-DE） gel electrophoresis condition for Candida albicans proteome analysis. Methods： Total cellular protein of Candida albicans strain SC5314 was extracted and .subjected to 2-DE under different conditions. The optimal conditions for sample preparation and 2-DE were screened by comparing the following parameters： cell broken methods, contents of lysis buffer, on-gel protein contents, and linear or non-linear immobilized pH gradient gels. Results： We successfully established the method for Candida albicuans protein sample preparation and optimized 2-DE condition for Candida albicans proteome analysis. A satisfactory 2-DE map with good repetition and resolution was obtained. Conclusion： The optimized condition of 2-DE in this article is suitable for Candida albicans strain, which paves a way for analysis of global protein profile in Candida albicans.