中文摘要：

目的探讨BDNF—TrkB信号传导通路与杏仁核电刺激点燃大鼠Arc表达及癫痫发生的关系。方法雄性sD大鼠随机分为空白组、假手术组、杏仁核点燃组（单纯点燃组、k252a注射组、DMSO对照组）。应用Mpha—Lab4通道信号采集及处理系统记录大鼠脑电活动。运用免疫组化检测其Arc蛋白的表达水平，采用RT—PCR、原位杂交检测ArcmRNA在海马中表达水平。结果k252a注射组大鼠V级发作持续时间（33．00±1．41）s显著低于单纯点燃组（60．50±2．07）s、DMSO对照组（60．00±1．79）s大鼠V级发作持续时间（P〈0．01）。k252a注射组3h、6hArc阳性细胞百分率显著低于单纯点燃组、DMSO对照组3h、6hArc阳性细胞百分率（P〈0．01），12h时5组间Arc阳性细胞百分率相比表达差异无统计学意义（F=0．684，P〉0．05）。RT—PCR、原位杂交结果显示：k252a注射组1h、3hArcmRNA表达量显著低于单纯点燃组、DMSO对照组1h、3hArcmRNA表达量（P〈0．01）。6h时5组间ArcmRNA相比表达差异无统计学意义（P〉0．05）。结论k252a能够抑制BDNF—TrkB信号传导通路对Arc表达的诱导作用从而使癫痫发作的持续时间缩短。

英文摘要：

Objective To study the relationship of BDNF - TrkB signaling pathway and the expression of Arc in amygdala electrical stimulation kindled rats and the epilepsy. Methods Male SD rats were divided randomly into control group, sham group and amygdala kindled group （simple kindling group, k252a injection group, DMSO control group ）. Application of Alpha - Lab 4 channel signal acquisition and processing system recording the EEG activity of rats. The expression level of Arc gene in hippocampus was test by RT - PCR and hybridization, The expression level of Arc protein was test by immunohistochemistry. Results The V class insultus duration of k252a injection group rats （33.00 ±1.41 ）s ,they were significantly less than the simple kindling group rats （60. 50 ± 2.07 ）s and the DMSO control group rats （60.00 ± 1.79）s （ P 〈 0. 0 1 ）. The Arc of positive cells percentage was significantly lower at 3 h,6 h in k252a injection group than simple kinding group, DMSO control group（ P 〈 0. 01 ）. The expression of Arc positive cells percentage among 5 groups at 12 h was no statistically significant （ F = 0. 684, P 〉 0. 05 ）. The resulted of RT -PCR, hybridization showing：The expression of Arc mRNA at 1 h, 3 hsignificantly lower in k252a injection group than simple kinding group,DMSO control group（ P 〈0. 01 ） . The defference of expression of Arc mRNA at 6 h among 5 groups was no statistically significant （ P 〉 0. 05）. Conclusion k252a can inhibiting BDNF - TrkB signaling pathyway inducing the expression of Arc, to reduce the duration of seizure.