中文摘要：

目的研究普罗布考（Probucol）在抗ox-LDL诱导THP-1巨噬细胞凋亡中对CD36、Caveolin-1表达的影响。方法用流式细胞仪检测细胞凋亡;用RT-PCR、免疫荧光分别检测CD36、Caveolin-1mRNA水平和蛋白表达。结果普罗布考抗ox-LDL诱导THP-1巨噬细胞凋亡;RT-PCR检测发现普罗布考组与ox-LDL组CD36、Caveolin-1mRNA表达无明显差异;免疫荧光检测结果显示普罗布考下调Caveolin-1蛋白表达,但对CD36蛋白的表达没有影响。结论普罗布考在抗ox-LDL诱导THP-1巨噬细胞凋亡中下调Caveolin-1蛋白的表达。

英文摘要：

Aim To study the effects of probucol on THP-1 macrophage apoptosis and CD36、Caveolin-1 expression induced by ox-LDL.Methods Apoptosis of THP-1 macrophages was determined by flow cytometry analysis.RT-PCR and immunofluorescence were used to detect CD36,Caveolin-1 mRNA level and protein expression respectively.Results Probucol had no effect on mRNA level of CD36,Caveolin-1 in THP-1 macrophages,but it attenuated Caveolin-1 protein expression.Conclusions Probucol can inhibit apoptosis induced by ox-LDL in THP-1 macrophages by down-regulating Caveolin-1 protein expression.