欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
纳米羟基磷灰石/壳聚糖复合材料（n-HA/CS）是目前骨修复替代领域使用广泛的一种生物材料.为探讨该材料在体内经代谢降解后,对大鼠体内代谢器官生理功能的影响,并分析其可能的作用机制.本研究用共沉淀法制备n-HA/CS复合材料,并采用红外光谱分析（IR）、X射线衍射（XRD）等手段表征该材料.通过细胞生长抑制实验,病理切片以及血清生化指标的检测,从体内体外两种方式观察该材料对MC3T3-E1细胞以及SD大鼠肝脏、肾脏等代谢器官的影响.结果显示,100 mg/kg含量n-HA/CS复合材料粉末在大鼠体内代谢8周,血清尿素氮（BUN）水平升高为29.6 mmol/L；肌酐（CR）水平升高为66.9 umol/L；总胆红素（T-B）及间接胆红素（I-B）水平也分别升高至1.79 umol/L和1.32 umol/L,均较对照组水平明显增高,有显著性差异（P≤0.05）.且肝脏、肾脏组织中出现大量凋亡细胞,无组织坏死和炎症表现.n-HA/CS复合材料在体内代谢8周后,通过诱导细胞凋亡影响机体肝、肾等代谢器官的功能.
英文摘要：
Composite conmposed of nano-hydroxyapatite and chitosan （n-HA/CS） is widely used in the field of bone repair and alternative. To investigate the degradation effects of n-HA/CS on metabolism organs in SD rat and analyze the possible mechanism, particulate n-HA/CS was prepared by precipitation and characterized by IR and XRD. Serum biochemical parameters measurement and histopathologieal analysis were performed to evaluate the composite toxicity after 8 weeks of composite implantation （100 mg/kg）. Results show that blood urea nitrogen （BUN） levels was 29.6 retool/L, creatinine （CR） levels was 66.9 umol/L, total bilirubin （T- B） and indirect bilirubin （I-B） levels were also increased to 1.79 umol/L and 1.32umol/L. Compared with that of control, there were significant differences （P~〈0.05）. A large number of apoptotic instead of necrosis cells were observed in the liver and renal proximal tubule tissue. It is concluded that the toxicity of n-HA/CS is attributable to the cells apoptosis.
同期刊论文项目
同项目期刊论文
The use of carbon nanotubes to induce osteogenic differentiation of human adipose-derived MSCs in vi
Bone and muscle structure and quality preserved by active versus passive muscle exercise on a new st
Influences of tensile load on in vitro degradation of an electrospun poly(L-lactide-co-glycolide) sc
Ultrasound-assisted fabrication of a biocompatible magnetic hydroxyapatite (in press, DOI: 10.1002/j
Biocompatible single-crystal selenium nanobelt based nanodevice as a temperature-tunable photosensor
Ultrasonic reflection coefficient and surface roughness index of OA articular cartilage: relation to
Relationship between triphasic mechanical properties of articular cartilage and osteoarthritic grade
Endothelium oriented differentiation of bone marrow mesenchymal stem cells under chemical and mechan
Effects of laterality, ankle inversion and stabilizers on the plantar pressure distribution during u
Effects of ankle eversion, limb laterality and ankle stabilizers on the transient postural stability
Evaluation on cartilage morphology after intra-articular injection of titanium dioxide nanoparticles
Flow patterns and wall shear stress distribution in human internal carotid arteries: The geometric e
Increased proliferation and differentiation of pre-osteoblasts MC3T3-E1 cells on nanostructured poly
Numerical simulation on the effects of drug-eluting stents with different links on hemodynamicsand d
Response of the authors on: Comments on "Effect of different labor forces on fetal skull molding" by
Emulsion self-assembly synthesis of chitosan/poly(lactic-co-glycolic acid) stimuli-responsive amphip
Comparative study of Newtonian and non-Newtonian simulations of drug transport in a model drug-eluti
Mechanotransduction of flow-induced shear stress by endothelial glycocalyx fibers is torque determin
Potential protection of green tea polyphenols against 1800 MHz electromagnetic radiation-induced inj
MICRO-FINITE ELEMENT ANALYSIS OF TRABECULAR BONE YIELD BEHAVIOR - EFFECTS OF TISSUE NONLINEAR MATERI
Osteogenic differentiation of human adipose-derived stem cells induced by osteoinductive calcium pho
Comparative study on measured variables and densitivity to bone microstructural changes induced by w
Stress analysis of first permanent mandibular molar with class 1 restorations of different cement ba
Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem c
Sustained release of VEGF by coaxial electrospun dextran/PLGA fibrous membranes in vascular tissue e
Kinematics, kinetics, and electromyogram of ankle during drop landing: A comparison between dominant
Micro-/nano-sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem
Improved hemocompatibility and endothelialization of vascular grafts by covalent immobilization of s
Biocomposites reinforced by fibers or tubes as scaffolds for tissue engineering or regenerative medi
Feedback system based on plantar pressure for monitoring toe-walking strides in children with cerebr
Preliminary animal studies on observation of injured spinal cord with intraoperative ultrasound back
Effect of cyclic strain on cardiomyogenic differentiation of rat bone marrow derived mesenchymal ste
期刊信息
位置：