中文摘要：

目的：探讨帕金森病（ PD）患者外周血T、B淋巴细胞亚群指标的变化规律，评估其作为PD诊断及病情评估的生物学标记的价值。方法以2013年3月至2014年6月从中山大学附属第一医院神经科门诊／住院77例PD患者及体检中心82名健康老年人为研究对象。流式细胞术检测研究对象外周血中CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD19＋淋巴细胞亚群百分比， t检验比较两组差异，多元线性回归分析PD组淋巴细胞亚群与其病程、病情严重程度及其药物剂量的相关性。结果PD患者外周血CD3＋、CD3＋CD4＋T淋巴细胞百分比较对照组明显降低[（62±12）％比（66±9）％， P＝0．04；（35±9）％比（38±7）％，P＝0．02]，尤其男性患者[（66±9）％比（61±13）％，P＝0．02；（38±10）％比（33±9）％，P＝0．01]。男性患者外周血CD3＋T淋巴细胞百分比与其病程呈正相关（ r＝0．329，P＝0．013，回归系数为1．423）。女性患者外周血CD3＋CD8＋T淋巴细胞百分比则与其病程呈负相关、与非运动症状评分呈正相关（r＝－0．309，P＝0．045；r＝0．370，P＝0．020；回归系数分别为－0．354，0．486）。结论 PD患者外周血的T淋巴细胞亚群比例失调，且存在性别差异。外周血淋巴细胞比例的变化可能有助于PD诊断及其病情进展的评估。

英文摘要：

Objective To explore the variations of subpopulation of peripheral lymphocytes in Parkinson′s disease （ PD ） and locate its potential biomarkers for clinical evaluations.Methods The methods of direct immunostaining and flow cytometry were employed to test the percentages of CD3 ＋, CD3 ＋CD4 ＋, CD3 ＋CD8 ＋, CD19 ＋lymphocytes in blood samples of 77 PD patients and 82 healthy controls.And the percentages of CD3 ＋CD4 ＋ and CD3 ＋CD8 ＋ lymphocytes and the parameters of patients and health controls were analyzed.Results Compared to controls, the percentages of CD3 ＋, CD3 ＋CD4 ＋lymphocytes significantly decreased in PD patients （ （62 ±12）%vs （66 ±9）%, P=0.04;（35 ±9）%vs （38 ±7）%, P=0.02）, especially in males （（66 ±9）%vs （61 ±13）%, P=0.02;（38 ±10）%vs （33 ±9）%, P=0.01））.Furthermore, the percentage of CD3 ＋lymphocytes had a positive correlation with the course of PD in male patients （ r =0.329, P =0.013, β=1.423 ） .And a negative correlation existed between the percentage of CD3 ＋CD4 ＋lymphocytes and the course of PD and there was a positive correlation with NMSS scale in female PD patients （r=-0.309, P=0.045, β=-0.354; r=0.370, P=0.020, β=0.486）. Conclusion The variants in subpopulation of peripheral lymphocytes in PD patients may serve as a potential biomarker for diagnosing PD and predicting its clinical course.