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中文摘要：
采用对个体连续采血的方法,研究了不同水温条件下氟苯尼考以30mg/kg的单剂量混饲口灌给药后在日本鳗鲡（Anguilla japonica）体内的药代动力学特征。利用DAS软件的统计矩原理计算每个个体的药时曲线关系,获得药动学参数,单因素方差分析结果表明,不同温度实验组间多个药动学参数存在显著性差异（P〈0.05）。20℃、24℃和28℃实验组药物峰浓度（Cmax）分别为（7.839±1.125）、（13.010±2.334）和（18.267±3.717）μg/mL,达峰时间（Tmax）分别为（6.500±2.070）、（4.500±1.414）和（3.429±0.926）h,这表明温度越高吸收越多越快。表观分布容积（Vz/F）分别为（3.964±0.594）、（2.466±0.672）和（1.841±0.485）L/kg,表明温度较高时氟苯尼考与血浆蛋白的结合更多。平均滞留时间（MRT0-∞）分别为（31.503±7.117）、（22.881±4.940）和（22.134±6.204）h,消除半衰期（t1/2z）分别为（21.243±5.166）、（14.994±4.293）和（14.656±5.061）h,24℃和28℃水温下的消除速率显著快于20℃实验组。药时曲线下面积（AUC0-∞）分别为（235.580±62.013）、（271.983±75.023）和（353.192±92.491）μg·h/mL,表明水温对相对生物利用度有显著影响。
英文摘要：
The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg/kg body weight to Japanese eel（Anguilla japonica） at the temperature of 20,24 and 28 ℃,respectively.Approximate 0.3 mL of blood sample from each eel in these three groups was collected in a row at the time 0.5,1,2,4,8,12,24 and 36 h after medicated-feed gavage.The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography（RP-HPLC）.The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software.Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software.It was found that most of parameters were different significantly between groups（P＆lt;0.05）.In the three groups at 20,24 and 28 ℃,pharmacokinetics parameters of peak plasma concentration（Cmax） were（7.839±1.125）,（13.010±2.334） and（18.267 ± 3.717） μg/mL,and the time to reach the Cmax（Tmax） were（6.500 ± 2.070）,（4.500 ± 1.414） and（3.429 ± 0.926） h,respectively.These suggested that eels at higher temperature absorbed more drug and more quickly.The volumes of distribution（Vz/F） were（3.964±0.594）,（2.466±0.672） and（1.841 ± 0.485） L/kg,respectively.The difference deduced that more florfenicol was bound to tissue in eels at lower temperature.The mean residence time（MRT0-∞） and the half-life of drug（t1/2z） in the three respective groups were（31.503 ± 7.117）,（22.881 ± 4.940） and（22.134±6.204） h,and（21.243 ± 5.166）,（14.994 ± 4.293） and（14.656 ± 5.061） h.These parameters showed that the elimination rate of florfenicol in eels at 24 ℃ or 28 ℃ was more quickly than that at 20 ℃.The areas under the concentration-time curve（AUC0-∞） were（235.580 ± 62.013）,（271.983 ± 75.023） and（353.192 ± 92.491） μg·h/mL,respective
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