为解决显微镜观测视野狭小的问题,提出了一种基于尺度不变特征变换(SIFT)特征的显微图像自动拼接方法。该方法充分考虑了显微图像采集过程以及显微图像自身的特点,通过一系列适用于显微图像拼接的算法,控制单幅图像拼接时间在1秒内,进而可以保证显微图像拼接随图像采集在线完成,同时,利用SIFF特征匹配准确度高的优点,可达到亚像素级的拼接精度。该方法拓展了显微镜的应用,尤其适用于生物和医学显微图像观测。
To solve the problem of narrow field of view in microscope, this paper proposes a new method of microscopic image stitching based on scale invariant feature transform (SIFT) features. This approach fully considers the properties of image acquisition and microscopic images and uses a series of algorithms suitable for microscopic image stitching, so it can limit its single inlage stitching time to 1 second. This means the on-line microscopic image stitching. For the high precision of the SIFT feature based matching, this approach can give the sub-pixel stitching accuracy.