中文摘要：

目的：检测抗PUF60抗体在中国特发性炎性肌病（IIM）患者中的阳性率,并分析其相关的临床意义。方法：本研究共纳入388例IIM患者[275例皮肌炎（DM）、76例多发性肌炎（PM）及37例肌炎-重叠综合征]和167例健康对照（HCs）。酶联免疫吸附测定（ELISA）测定入组患者及健康对照的抗体滴度,并联合免疫印迹实验验证ELISA结果的可靠性。结果：抗PUF60抗体可见于各IIM亚组,阳性率均高于健康对照组,包括11.3%（P〈0.01）的DM、7.9%（P〈0.05）的PM和21.6%（P〈0.01）的肌炎重叠综合征患者。在经典DM中,该抗体常见于肌炎抗体阴性的患者（P〈0.05）,并与技工手发生相关;且该抗体在无肌病性皮肌炎中阳性率较高,与皮肤溃疡的发生相关。结论：抗PUF60抗体在中国肌炎患者中普遍存在,且不同亚型的临床相关性各异,提示抗PUF60抗体或其靶抗原PUF60可能在不同亚型的肌炎中参与了不同的致病过程。

英文摘要：

Objective：To detect the existence and to analyze the clinical significance of anti-PUF60 antibody in Chinese patients with idiopathic inflammatory myopathy（IIM）.Methods：This study involved 388 IIM patients [including 275 patients with dermatomyositis （DM）,76 with polymyositis （PM）and 37 with myositis overlap syndrome] and 167 healthy controls（HCs）.An ELISA was developed to detect serum anti-PUF60 antibody and immunoblotting analyses were performed to validate the ELISA results.Results：Anti-PUF60 antibody presented in 11.3% DM patients,7.9% PM patients and 21.6% patients with myositis overlap syndrome,and the antibody positive rates of them were significantly higher than HCs （P〈O.05,P〈0.01）.Classical DM patients with anti- PUF60 antibody had lower prevalence of currently known myositis antibodies（P〈0.OS）and higher prevalence of mechanic＇s hands.While the prevalence of anti-PUF60 antibody was particularly higher in the CADM subgroup （29%）and associated with the incidence of skin ulcer.Conclusion：Anti-PUF60 antibody can be detected in Chinese patients with IIM,and it is associated with distinct clinical features in different rheumatic diseases.This finding indicates that anti-PUF60 antibody is associated with different pathogenesis processes in different IIM subgroups.