中文摘要：

用去分化的成熟脂肪细胞建立一种新的具有再增殖和再分化能力的猪前体脂肪细胞模型.用“天花板”培养法分离、培养1～3日龄仔猪皮下成熟脂肪细胞,显微镜下观察细胞形态变化并计数,流式细胞术检测细胞周期;油红O染色法检测脂肪细胞分化率,RT-PCR分析前体脂肪细胞标志基因Pref-1及成熟脂肪细胞关键转录因子PPARγ和C/EBPα等mRNA表达情况.发现刚贴壁的细胞为单室脂滴成熟脂肪细胞,油红O染色完全阳性;14d后这种成熟脂肪细胞完全去分化为无脂滴的纤维状细胞,并表达前体脂肪细胞标志基因Pref-1,油红O染色阴性.这种去分化的前体脂肪细胞在成脂诱导剂作用下,可重新分化为成熟的脂肪细胞.结果证实,成熟脂肪细胞去分化后的前体脂肪细胞可重新增殖、分化为成熟脂肪细胞,是一种新的有效的前体脂肪细胞模型.

英文摘要：

The adipose tissue isolated from the 1- 3 day piglets subcutaneouly, and mature adipocytes were cultured by ＂ceiling culture＂ method. Morphological changes in different culture days were observed, cell proliferation was measured by cell count method, and the cycles were analyzed by flow cytometry （FCM）. Via oil red O staining assess the degree of adipogenesis and differentiation was assessed, the gene expression of preadipocyte factor- 1 （Pref- 1 ）, peroxisome proliferator-activated receptor-γ （PPARγ） and CCAAT/enhancer binding protein-α （C/EBPα） was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. The results showed that the primal adherences ceils were mature adipocytes with imple lipids, and completed positive with oil red O staining, the mature adipocytes de-differentiated to fiber cell without any lipids after 14 days. The fiber cells expressed preadipocytes marker Pref-1 but not myogenic regulatory protein （MyoD）, and negative with oil red O staining. The de-differentiated fiber cell can redifferentiated into mature adipocytes with lipids, and express adipocytes markers PPAR-γ and C/EBPa when cultured in adipogenesis inducer mediums. The results indicate that the de-differentiated preadipocytes can redifferentiated into mature adipocytes with ability of proliferation, and it may be a valid preadipocyte cell model.