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中文摘要：
根据禽网状内皮组织增生症病毒SNV株的前病毒基因组cDNA序列设计并合成1对引物,以pPB101质粒为模板,通过PCR扩增获得该病毒p30基因片段。将PCR产物克隆入表达载体pCold-HF中,构建的原核表达质粒pCold-p30转化BL21（DE3）菌,经IPTG诱导,p30蛋白呈现可溶性表达。用表达产物免疫6周龄BALB/c小鼠,制备p30抗体,经Westernblot和间接免疫荧光法检测,结果发现,原核表达的SNV株p30蛋白具有良好的免疫原性。
英文摘要：
In the study, p30 gene of Avian reticuloendotheliosis virus （REV） was amplified by PCR from provirus genomic cDNA clone of SNV strain. The p30 gene was then subcloned into the pCold-HF vector. The resulting recombinant plasmid （pCP30） was transformed into the competent cells BL21 （DE3）. With induction of isopropylthio-13-D-galactoside （IPTG）, soluble p30 protein was expressed in BL21 cells and visualized as a band of 72 kDa in SDS-PAGE. Six-week old BALB/c mice were immunized with the purified protein to prepare p30 antiserum. The reactivity of the antiserum was determined in Western blot and indirect immunofluorescent assay. The availability of p30 antiserum would benefit to develop diagnostic method for the detection of REV infection in chickens and waterfowls.
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The Mycoplasma gallisepticum alpha-enolase is cell surface-exposed and mediates adherence by binding
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