中文摘要：

文章介绍了一种密度梯度离心的方法提取刺槐线粒体。该线粒体提取方法包括以下步骤：（1）材料的研磨，获取匀浆：（2）差速离心去除细胞碎片等杂质；（3）加悬浮液，密度梯度获取较纯的线粒体沉淀。结果表明，采用这种方法得到的线粒体完整性较好。可以用于线粒体功能的研究，以及为线粒体蛋白质组学的研究奠定基础。

英文摘要：

A method for the isolated and sub-fractionation of the mitoehondria of Robinia pseudoacacia from plant tissue is described. The method comprised：（1） the blender tissue homogenization and the homogenate of tissue was obtained;（2） different centrifugation to remove impurities such as cell fragments;（3） add suspension, then, density gradient to obtain relatively pure mitochondria precipitation. Results showed that the integrity of mitochondrial was better by using this method and suitable for study on mitochondrial function as well as lay the foundation for the study of mitoehondrial proteomics.